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DOI :
10.3791/53939-v
•
12:37 min
April 14th, 2016
Chapters
0:05
Title
0:57
Extraction of Total Nucleic Acid from Cervicovaginal Swabs (Phenol-Chloroform Extraction)
5:52
PCR Amplification of the 16S rRNA Gene V4 Hypervariable Region
9:40
Library Pooling and High-Throughput Sequencing
10:30
Evaluation of Nucleic Acid Extraction and 16S rRNA Gene PCR Amplification
11:35
Conclusion
我们描述了从拭子用16S rRNA基因扩增子测序细菌群落特征提取核酸的高效,可靠和成本有效的方法。该方法允许对多种样品类型共同的处理方法,并容纳许多下游分析流程。
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