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DOI :
10.3791/53978-v
June 21st, 2016
Chapters
0:05
Title
0:45
Enhancer Selection Based on ChIP-seq Analysis
1:27
Transfection of Embryonic Stem Cells
3:21
β-Galactosidase and Luciferase Assays
5:19
Characterization of Enhancer RNA and Measurement of eRNA Transcription
6:34
Results: Retinoic Acid Induced Enhancer Activation in Embryonic Stem Cells
8:13
Conclusion
In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.
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