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DOI :
10.3791/53983-v
May 30th, 2016
Chapters
0:05
Title
0:43
Heart Isolation and Cannulation
4:00
Digestion and Vascular Smooth Muscle Cell (VSMC) Isolation
6:08
VSMC Culture
7:10
Results: Representative Cultured Coronary VSMC
7:58
Conclusion
このプロトコルの目的は、冠循環からマウスの一次血管平滑筋細胞(VSMC)の単離および培養技術を実証することです。たVSMCが単離されたら、それらは、多くの標準的な培養技術のために使用することができます。
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