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DOI :
10.3791/53992-v
November 27th, 2016
Chapters
0:05
Title
1:48
Preparing the CaspaseTracker Flies and Dissected Tissues
4:30
Tissue Staining and Mounting
8:11
Confocal Microscopy
9:37
Results: Caspase Biosensor Activity in Non-dying Cells of Healthy Flies
12:10
Conclusion
カスパーゼ活性の低いレベルを含む全動物に健康な細胞を検出するために、CaspaseTracker指定された高感度バイオセンサーは、 ショウジョウバエのために生成されました。カスパーゼ依存性のバイオセンサーの活性は、死刺激の非存在下で最適化された条件下で飼育した成体動物の臓器全体長寿命健康な細胞で検出されます。
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