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JoVE Journal

Bioengineering

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

10.3K Views

10:21 min

May 5th, 2016

May 5th, 2016

10,332 Views

0:05

Title

0:34

Master Fabrication

3:42

PDMS Device Fabrication

5:24

Preparation of Optical Components

6:41

Optical Fiber Insertion

7:33

Fluorescence Detection of Mixed Emulsions

8:53

Results: Sensitive Detection of Fluorescent Droplets Using Optical Fibers

9:55

Conclusion

Transcript

The overall goal of this procedure is to demonstrate a simple application of droplet microfluidics fluorescence detection using optical fibers. This method can help answer key questions in high throughput biological screening, such as the identifi

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Multicolor fluorescence detection in droplet microfluidics typically involves bulky and complex epifluorescence microscope-based detection systems. Here we describe a compact and modular multicolor detection scheme that utilizes an array of optical fibers to temporally encode multicolor data collected by a single photodetector.

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