Name: primary outcome assessment in a pig model of acute myocardial infarction
Uploaded: 2016-10-14T15:00:00
Description: Watch this Scientific Journal Video about Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction at JoVE.com

The authors gratefully acknowledge Marlijn Jansen, Joyce Visser, Grace Croft, Martijn van Nieuwburg, Danny Elbersen and Evelyn Velema for their excellent technical support during the animal experiments.

Tüm hayvan deneyleri Utrecht Üniversitesi Tıp Merkezi Hayvan Deneyleri Etik Komitesi (Utrecht, Hollanda) tarafından onaylanan ve &#39;laboratuvar hayvanlarının bakımı ve kullanımı için Kılavuzu&#39; uygun bulundu. NOT: Protokol kapalı göğüs balon oklüzyon mevcut yazının bir parçası değildir ve başka bir yerde 5 ayrıntılı olarak tarif edilmiştir gerçekleştirmek için. Kısacası, domuz (60-70 kg) sol ön inen arter (LAD) orta kısmı 75 dk translüminal...

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Kardiyak biçimlenme büyük ölçüde miyokard infarktüsü boyutuna bağlı olarak ve miyokard enfarktüsü kalitesi 6,26 onarın. Standart bir şekilde önceki değerlendirmek için, bu yazıda onaylanmış ve yoğun 8,16,27,28 kullanılmıştır eks vivo TTC lekelemesi ile kombine Evans mavisi in vivo enfüzyonu, zarif bir yöntem sağlar. Bu yöntem, AAR 16 ilgili risk (AAR) de alanın ölçümü ve enfarktüs boyutuna izin verir. miyokard ponksiyon gerektirmez ola...

Batı dünyasında 1 insanların% 2 - azaltılmış ejeksiyon fraksiyonu (HFrEF) ile kalp yetmezliği tahmini 1 etkileyen, tüm kalp yetmezliği vakaların yaklaşık% 50&#39;sini oluşturmaktadır. Onun en yaygın nedeni akut miyokard infarktüsü (AMI) &#39;dir. AMI sonrası akut mortalite artan farkındalık ve gelişmiş tedavi seçenekleri önemli ölçüde azalmıştır gibi, vurgu kronik sekel doğru kaymıştır; En önemli varlık HFrEF 2,3. Birlikte sağlık maliyetlerinden 4 artan, kalp yetmezliği artan salgın daha önce 5 açıklandığı gibi AMI sonrası olumsuz biçimlenme çok öteleme domuz modelinde ele alınabilir yeni teşhis ve tedaviler için ihtiyaç, vurgulamaktadır. Olumsuz yeniden şekillenme Hem belirleyicileri (örneğin, enfarktüs boyutu) ve fonksiyonel değerlendirmeler (örneğin, ekokardiyografi) sıklıkla rel ihtiyacını belirten yeni terapötik etkinliği testi için kullanılıriable ve nispeten ucuz yöntemler. Bu yazının amacı, akut miyokard infarktüsü domuz modelinde etkinlik testleri için önemli ve güvenilir sonuç tedbirler bu ihtiyacı hitap etmektir. Bu riski (AAR), 3D transözofajiyal ekokardiyografi (3D-TEE) ve ayrıntılı giriş bazlı basınç-hacim (PV) döngü edinimi de bölgeye ilişkin enfarktüs boyutunu (IS) içerir. Enfarktüs boyutu AMI 6 sonra olumsuz yeniden ve hayatta kalma ana belirleyicisidir. Tersinir yaralı kardiyomiyositlerde kurtarma ve enfarktüs boyutunu sınırlayabilir iskemik miyokard zamanında reperfüzyon, reperfüzyon kendisi oksidatif stresin üretimi ve orantısız bir inflamatuvar yanıt ile ek hasara neden olsa da (iskemi reperfüzyon hasarı (IRI)) 7. Bu nedenle, IRI gelecek vaat eden bir terapötik hedef olarak tanımlanmıştır. infarkt boyutunu azaltmak için yeni tedavilerin yeteneği ile ilgili olarak değerlendirerek enfarktüs boyutuna göre ölçülürriskli alan (AAR) için. Daha büyük bir AAR daha büyük bir mutlak enfarkt boyutu neden olarak AAR miktar, hayvan modellerinde koroner anatomi bireyler arası değişkenliğin düzeltmek için zorunludur. Enfarktüs boyutu doğrudan kardiyak performans ve miyokard kontraktilite ile ilgili olduğundan, AAR değişimler tedavi yaklaşımları 8 bakılmaksızın sonuç önlemleri incelemek etkileyebilir. Üç boyutlu transözofajiyal ekokardiyografi (3D-TEE) kardiyak fonksiyon non-invaziv ölçmek için bir klinik uygulanabilir, en önemlisi, güvenli, güvenilir ve ucuz bir yöntemdir. Transtorasik ekokardiyografi (TTE) görüntüleri domuzlarda 9 2D parasternal uzun ve kısa eksen kesitlerinde sınırlıdır oysa, 3D-TEE sol ventrikül tam 3 boyutlu görüntü elde etmek için kullanılabilir. Bu nedenle, bu tür modifiye Simpson kuralına 10 olarak sol ventrikül (LV) ciltlik Matematiksel yaklaşımlar gerekmez. İkincisi Düzeltici gerisindedirectly nedeniyle silindirik geometri 11 eksikliği LV yeniden şekillenme sonra LV hacimleri tahmin. Mevcut modelde 12 kardiyoprotektif etkiler yarattığı gözlenmektedir cerrahi müdahale gerektirmez Dahası, 3D-TEE epikardiyal ekokardiyografi üzerinde tercih edilir. Miyokardiyal fonksiyon değerlendirme 2B T şeklindeki kullanımı 13,14 daha önce tarif edilmiş olmasına rağmen, ventriküler geometrisi ile ilgili sınırlamalar 2D TTE gözlenenlere benzer ve AG yeniden ölçüde bağlıdır. Bu nedenle, büyük enfarktüs (ve dolayısıyla kalp yetmezliği olasılığı daha yüksek), daha büyük olasılıkla 2B ölçümleri yanlış geometrik varsayımlar tarafından kusurlu hale gelir ve 3D teknikleri için daha yüksek gerekir. Bununla birlikte, çoğu görüntüleme yöntemleri miyokard içsel fonksiyonel özelliklerini değerlendirmek için kendi yeteneği sınırlıdır. PV gibi ilgili ek bilgi sağlayabilir döngüler ve onların satın alma nedenleAşağıda detaylı bir şekilde tarif.

<table border="0" cellpadding="0" cellspacing="0" width="875">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">3-dimensional transesophageal echocardiography</td>
			<td style="width:180px;"></td>
			<td style="width:108px;"></td>
			<td style="width:191px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">iE33 ultrasound device</td>
			<td style="width:180px;">Philips</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">X7-2t transducer</td>
			<td style="width:180px;">Philips</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Aquasonic&#174; 100 ultrasound transmission gel</td>
			<td style="width:180px;">Parker Laboratories Inc.</td>
			<td style="width:108px;">01-34</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Battery handle type C (laryngoscope handle)</td>
			<td style="width:180px;">Riester</td>
			<td style="width:108px;">12303</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Ri-Standard Miller blade MIL 4 (laryngoscope blade)</td>
			<td style="width:180px;">Riester</td>
			<td style="width:108px;">12225</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Qlab 10.0 (3DQ Advanced) analysis software</td>
			<td style="width:180px;">Philips</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Pressure-volume loop acquisition</td>
			<td style="width:180px;"></td>
			<td style="width:108px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Cardiac defibrillator</td>
			<td style="width:180px;">Philips</td>
			<td style="width:108px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">0.9% saline</td>
			<td style="width:180px;">Braun</td>
			<td style="width:108px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">8F Percutaneous Sheath Introducer Set</td>
			<td style="width:180px;">Arrow</td>
			<td style="width:108px;">CP-08803</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">9F Radifocus&#174; Introducer II Standard Kit&#160;</td>
			<td style="width:180px;">Terumo</td>
			<td>RS*A90K10SQ</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">8F Fogarty catheter</td>
			<td style="width:180px;">Edward Life Sciences</td>
			<td>62080814F</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">7F Criticath&#8482; SP5107H TD catheter (Swan-Ganz)</td>
			<td style="width:180px;">Becton Dickinson (BD)</td>
			<td>680078</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:397px;">Ultraview SL Patient Monitor and Invasive Command Module (external cardiac output device)</td>
			<td>Spacelabs Healthcare</td>
			<td>91387</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">ADVantage system&#8482;</td>
			<td>Transonic SciSense</td>
			<td>-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">7F tetra-polar admittance catheter (7.0 VSL Pigtail / no lumen)</td>
			<td>Transonic SciSense</td>
			<td>-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Multi-channel acquisition system (Iworx 404)</td>
			<td>Iworx</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Labscribe V2.0 analysis software</td>
			<td style="width:180px;">Iworx</td>
			<td style="width:108px;">-</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Infarct size / area-at-risk quantification</td>
			<td style="width:180px;"></td>
			<td style="width:108px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Diathermy</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;"></td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Lebsch knife</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;"></td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Hammer</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;"></td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Bone marrow wax</td>
			<td style="width:180px;">Syneture</td>
			<td style="width:108px;"></td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Klinkenberg scissors</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;"></td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Retractor</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;"></td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Surgical scissors</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">7F Percutaneous Sheath Introducer Set&#160;</td>
			<td style="width:180px;">Arrow</td>
			<td>CP-08703</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">8F Percutaneous Sheath Introducer Set&#160;</td>
			<td style="width:180px;">Arrow</td>
			<td style="width:108px;">CP-08803</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">7F JL4 guiding catheter&#160;</td>
			<td style="width:180px;">Boston Scientific</td>
			<td style="width:108px;">H749 34357-662</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">8F JL4 guiding catheter&#160;</td>
			<td style="width:180px;">Boston Scientific</td>
			<td>H749 34358-662&#160;</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">COPILOT Bleedback Control Valves&#160;</td>
			<td>Abbott Vascular</td>
			<td>1003331</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">BD Connecta&#8482;&#160;</td>
			<td style="width:180px;">Franklin Lakes</td>
			<td>394995</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Contrast agent</td>
			<td style="width:180px;">Telebrix</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:397px;">Persuader 9 Steerable Guidewire 9 (0.014&#34;, 180 cm, straight tip), hydrophilic coating</td>
			<td style="width:180px;">Medtronic Inc.</td>
			<td>9PSDR180HS</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:397px;">SAPPHIRE&#8482; Coronary Dilatation Catheter (PTCA balloon suitable for the size of the particular coronary artery (2.75 - 3.25 mm))</td>
			<td style="width:180px;">OrbusNeich</td>
			<td style="width:108px;">103-3015</td>
			<td>Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Evans Blue&#160;</td>
			<td style="width:180px;">Sigma-Aldrich</td>
			<td>E2129-100G</td>
			<td>Toxic. Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">2,3,5-triphenyl-tetrazolium chloride (TTC)</td>
			<td style="width:180px;">Sigma-Aldrich</td>
			<td>T8877-100G</td>
			<td>Irritant. Alternative product can be used</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">9V battery</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Ruler</td>
			<td style="width:180px;">-</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:397px;">Photocamera</td>
			<td style="width:180px;">Sony</td>
			<td style="width:108px;">-</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:397px;">ImageJ</td>
			<td style="width:180px;">National Institutes of Health</td>
			<td style="width:108px;">-</td>
			<td>Alternative product can be used</td>
		</tr>
	</tbody>
</table>

primary outcome assessment in a pig model of acute myocardial infarction

Mortality after acute myocardial infarction remains substantial and is associated with significant morbidity, like heart failure. Novel therapeutics are therefore required to confine cardiac damage, promote survival and reduce the disease burden of heart failure. Large animal experiments are an essential part in the translational process from experimental to clinical therapies. To optimize clinical translation, robust and representative outcome measures are mandatory. The present manuscript aims to address this need by describing the assessment of three clinically relevant outcome modalities in a pig acute myocardial infarction (AMI) model: infarct size in relation to area at risk (IS/AAR) staining, 3-dimensional transesophageal echocardiography (TEE) and admittance-based pressure-volume (PV) loops. Infarct size is the main determinant driving the transition from AMI to heart failure and can be quantified by IS/AAR staining. Echocardiography is a reliable and robust tool in the assessment of global and regional cardiac function in clinical cardiology. Here, a method for three-dimensional transesophageal echocardiography (3D-TEE) in pigs is provided. Extensive insight into cardiac performance can be obtained by admittance-based pressure-volume (PV) loops, including intrinsic parameters of myocardial function that are pre- and afterload independent. Combined with a clinically feasible experimental study protocol, these outcome measures provide researchers with essential information to determine whether novel therapeutic strategies could yield promising targets for future testing in clinical studies.

<html> 3D Transözofageal Ekokardiyografi 3D transözofageal ekokardiyografi (3D-TEE), küresel kalp fonksiyonunun değerlendirilmesi için kullanılabilir. AMI sonrası, küresel kalp fonksiyonu sağlıklı başlangıç ​​değerlerine farklıdır. Özellikle, sol ventrikül ejeksiyon fraksiyonu (LVEF) reperfüzyon bir hafta (n = 10) (GPJ van Hout, 2015) sonra 37 ±% 6 59 ±% 4 azalır. diyastol sonu hacim ...

Watch this Scientific Journal Video about Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction at JoVE.com

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

Güvenilir ve doğru sonuç değerlendirmesi klinik tedavi içine klinik öncesi tedavilerin çeviri için anahtardır. Geçerli kağıt domuz akut miyokard infarktüsü modelinde kardiyak performans ve hasar üç klinik açıdan primer sonuç parametreleri değerlendirmek açıklamaktadır.

Akut Miyokard İnfarktüsü bir Domuz Modeli İlköğretim Sonuç Değerlendirmesi

Mortality after acute myocardial infarction remains substantial and is associated with significant morbidity, like heart failure. Novel therapeutics are ...

Research

JoVE Journal

Medicine

Acute Myocardial Infarction in Rats

Sıçanlarda Akut Miyokard Enfarktüsü

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

MRI and PET in Mouse Models of Myocardial Infarction

Myocardial infarction is one of the leading causes of death in the Western world. The similarity of the mouse heart to the human heart has made it an ...

Myocardial Infarction and Functional Outcome Assessment in Pigs

Domuzlar miyokard enfarktüsü ve Fonksiyonel Sonuç Değerlendirmesi

Introduction of newly discovered cardiovascular therapeutics into first-in-man trials depends on a strictly regulated ethical and legal roadmap. One ...

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

Yenidoğan Farelerde Miyokard Enfarktüsü, Kalp Rejenerasyon Bir Model

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and ...

Real-time Pressure-volume Analysis of Acute Myocardial Infarction in Mice

Akut miyokard infarktüsünde farelerde gerçek zamanlı basınç-hacim Analizi

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any ...

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Akut miyokard infarktüsü ve kardiyovasküler hastalık mikroRNA'lar dolaşan miktarının için dijital PCR

Circulating serum microRNAs (miRNAs) have shown promise as biomarkers for the cardiovascular disease and acute myocardial infarction (AMI), being released ...

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

HiPSC elde edilen kalp kası yamalar implantasyonu eskiden şiling şimdi domuz modelinde miyokardiyal yaralanma sonra

Due to the limited regeneration capacity of the heart in adult mammals, myocardial infarction results in an irreversible loss of cardiomyocytes. This loss ...

A Cryoinjury Model to Study Myocardial Infarction in the Mouse

Farede Miyokard Enfarktüsü Çalışması İçin Kriyoyaralanma Modeli

The use of animal models is essential for developing new therapeutic strategies for acute coronary syndrome and its complications. In this article, we ...

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

Murine Modelinde Miyokard İnfarktüsünden Sonra MSC Yüklemeli Enjekte Edilebilir Hidrojellerin intramiyokardiyal Transplantasyonu

One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of ...

Utilizing Percutaneous Ventricular Assist Devices in Acute Myocardial Infarction Complicated by Cardiogenic Shock

Kardiyojenik Şokun Karmaşıklaştırdığı Akut Miyokard Enfarktüsünde Perkütan Ventrikül Destek Cihazlarının Kullanılması

Cardiogenic shock is defined as persistent hypotension, accompanied by evidence of end organ hypo-perfusion. Percutaneous ventricular assist devices ...