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DOI :
10.3791/54024-v
June 15th, 2016
Chapters
0:05
Title
0:59
Slice Placement and Visualization
2:15
Micropipette Placement and Positioning
3:45
Final Approach, Seal Formation, and Obtaining the Whole-cell Configuration
5:56
Results: Effect of Temperature, Series Resistance, and Input Resistance on the Waveform of the Electrical Signal
6:47
Conclusion
这个协议描述用于执行全细胞膜片钳记录基本程序步骤。这种技术允许神经元的电行为的研究,并在大脑切片进行时,允许的各种神经元功能从仍在集成在相对保存良好脑电路的神经元的评估。
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