Name: an orthotopic mouse model of spontaneous breast cancer metastasis
Uploaded: 2016-08-14T13:00:00
Description: Watch this Scientific Journal Video about An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis at JoVE.com

Supported by grants from the National Institute of Health grants CA133085, CA182518 and CA185909 (to KL) and VA Merit Review Award BX001962 (to KL).

所有的程序遵循摄政佐治亚大学动物使用及护理委员会指导原则和批准协议。 1.原位乳腺癌肿瘤的建立<ol><li>实验前一天，培养约2×10 6个 4T1-吕克肿瘤细胞在10厘米的培养皿在10ml RPMI培养基中含有10％FBS。孵育在CO 2培养箱中的培养皿，在37℃和5％的CO 2。 </li><li>对肿瘤细胞注射当天，从与真空培养皿除去培养基，加入5毫升无菌磷酸盐缓冲盐水（PBS），pH为7.4至培养皿...

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许多类型的乳腺癌转移的转基因小鼠模型已经被开发。1这些转基因小鼠具有高的肿瘤的发病率范围从60到100％。然而，这些转基因小鼠的转移发生率比肿瘤发病率要低得多（14 - 100％）。肿瘤细胞转移至LN和肺中的大多数乳腺癌转移的这些转基因小鼠模型。转移延迟从模型变化以模拟和从2〜8个月的范围内。6,11-16乳腺癌转移的这些转基因小鼠模型可用于研究乳腺癌转移基础的遗传...

根据美国癌症协会，乳腺癌是女性癌症在美国是最常见的诊断形式。在最近开发的靶向疗法组合早期检测已经显著减少乳腺癌的死亡率在过去二十年。然而，乳腺癌仍然是妇女在美国1癌症相关死亡的第二大原因。大多数乳腺癌患者的死亡是由于肿瘤细胞转移。不幸的是，大多数的乳腺癌是浸润性和经常转移至淋巴结，并随后到远处器官，包括骨，肺，肝和脑。1,2-目前转移性乳腺癌没有有效的治疗方法。因此，化疗和免疫治疗剂的发展来抑制转移性乳腺癌具有十分重要的意义。 各种乳腺癌自发转移小鼠模型bEEN开发研究乳腺肿瘤细胞进展和转移，也可以用作模型治疗剂的发展的分子机制。1,3-5然而，大多数这些小鼠模型是遗传肿瘤模型，虽然优良模型机械研究，不适合用于测试治疗剂，因为转移需要几个月在这些遗传模型来开发，因此需要的抗癌剂的昂贵长期施用。在活体小鼠6,7-监测肿瘤进展也是技术上具有挑战性。相反，移植乳腺癌转移模型具有短期肿瘤进展和在活体小鼠的肿瘤进展的容易跟踪的优点。在4T1原位乳腺癌自发转移的小鼠模型是这样的移植肿瘤模型8在这个模型中，乳腺肿瘤细胞移植到乳房脂肪垫建立原发性肿瘤结节。原发性肿瘤然后可在人类乳腺癌患者进行手术切除。 4T1肿瘤细胞是高度侵入性的。8,9，3,10几乎所有携带肿瘤的小鼠发展转移在30天后肿瘤移植到乳房脂肪垫后。然而，4T1肿瘤的原发部位长出积极与肿瘤大小往往超出了允许的大多数动物协议的限制。在这个阶段中，转移往往是微转移。因此，重要的是去除原发肿瘤，以允许用于测试治疗剂转移的进展。这里，我们报告建立原发性肿瘤移植，手术切除原发肿瘤和4T1肿瘤的生长和发展在体内的生物发光基于成像的量化的一个简单且敏感的程序。

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an orthotopic mouse model of spontaneous breast cancer metastasis

转移是乳腺癌患者的死亡的首要原因。该机制癌细胞转移，包括乳腺癌转移底层，在很大程度上是未知的，是在癌症研究的焦点。各种乳腺癌自发转移小鼠模型已经建立。在这里，我们报告的简化程序，建立原位移植乳腺癌原发灶和由此产生的自发转移，模仿人类乳腺癌转移。用生物发光活肿瘤成像相结合，该小鼠模型允许肿瘤生长和进展动力学进行监测和定量。在这种模式下，4T1-吕克乳腺癌细胞的低剂量（1×10 4个细胞），使用结核菌素注射器注入BALB / c小鼠乳腺脂肪垫。小鼠用萤光素注射，并在使用生物发光成像系统的不同时间点成像。当原发肿瘤生长的大小限制为在IACUC批准的协议（APPRoximately 30天），将小鼠在2％异氟烷和氧气的流量恒定麻醉。肿瘤区域用70％乙醇灭菌。肿瘤周围的小鼠皮肤切除以暴露其用一对无菌剪刀除去肿瘤。原发肿瘤的切除延伸的4T-1荷瘤小鼠的存活一个月。小鼠然后重复成像的转移性肿瘤扩散到远处器官。治疗剂可施用在这一点上，以抑制肿瘤的转移。这种模式是在主站点和进展动力学远处器官量化乳腺癌细胞的生长简单而敏感，因此是研究乳腺癌的生长和恶化，并在体内测试抗转移治疗和免疫治疗剂的优秀典范。

原位乳腺癌小鼠模型的建立 4T1是一个积极的乳腺癌细胞系。小至1×10 4个细胞进入乳房脂肪垫的注射可导致建立一个单一的肿瘤结节中注入（ 图2A）的部位。因此，肿瘤模仿人原发性乳房癌。几乎100％的小鼠发展的原位肿瘤。肿瘤大小可以使用数字测径器或通过活肿瘤成像（ 图3）进行定量。肿瘤是使用生物发光成像系?...

Watch this Scientific Journal Video about An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis at JoVE.com

An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

原位乳腺癌原发肿瘤模型和手术切除原发肿瘤，延长生命鼠标产生自发转移的描述。肿瘤的生长和发展进行监测和通过萤光素酶荧光成像定量。

自发性乳腺癌转移的原位小鼠模型

Metastasis is the primary cause of mortality of breast cancer patients. The mechanism underlying cancer cell metastasis, including breast cancer ...

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An Orthotopic Model of Murine Bladder Cancer

一个小鼠膀胱癌原位模型

In this straightforward procedure, bladder tumors are established in female C57 mice through the use of catheterization, local cauterization, and ...

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Quantitative Analysis of Cancer Metastasis using an Avian Embryo Model

癌症转移，使用禽流感胚胎模型的定量分析

During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex ...

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

多光子成像在口腔鳞状细胞癌原位小鼠模型的肿瘤细胞的侵袭

Loco-regional invasion of head and neck cancer is linked to metastatic risk and presents a difficult challenge in designing and implementing patient ...

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model

在小鼠模型体内生物发光成像乳腺癌脑转移的肿瘤乏氧动力学

It is well recognized that tumor hypoxia plays an important role in promoting malignant progression and affecting therapeutic response negatively. There ...

Combination Radiotherapy in an Orthotopic Mouse Brain Tumor Model

在原位小鼠脑肿瘤模型结合放疗

Glioblastoma multiforme (GBM) are the most common and aggressive adult primary brain tumors1. In recent years there has been substantial progress in the ...

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma

甲状腺未分化癌的原位小鼠模型

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原位膀胱癌模型的基因传递研究

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Bioluminescent Orthotopic Model of Pancreatic Cancer Progression

生物荧光原位模型胰腺癌进展

Pancreatic cancer has an extremely poor five-year survival rate of 4-6%.  New therapeutic options are critically needed and depend on improved ...

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

人类前列腺癌转移的原位小鼠模型

Our laboratory has developed a novel orthotopic implantation model of  human prostate cancer (PCa). As PCa death is not due to the primary tumor, but  ...

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

乳腺癌的一种新的转基因小鼠模型：转移性乳腺癌通过瞄准导管上皮与腺病毒Cre重组酶的启动

Breast cancer is a heterogeneous disease involving complex cellular interactions between the developing tumor and immune system, eventually resulting in ...