Name: assessment of cardiac morphological and functional changes in mouse model of transverse aortic constriction by echocardiographic imaging
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Description: Watch this Scientific Journal Video about Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging at JoVE.com

The authors have nothing to disclose.

يتبع بروتوكول للمبادئ التوجيهية للجنة رعاية واستخدام الحيوان المؤسسي من جامعة واشنطن. 1. إجراء العمليات الجراحية والتحضير للتصوير <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> يخضع C57BL / 6 الفئران إلى معاهدة الصداقة والتعاون ...

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وقد استخدم على نطاق واسع تخطيط صدى القلب لتقييم وظيفة القلب في نماذج القوارض من 2،6 أمراض القلب. مقارنة منهجيات الغازية أو الطرفية مثل حجم الضغط قياس حلقة 11 وخارج الحي العمل القلب 12، ويوفر تخطيط صدى القلب قوية وأداة موسع لتقييم التغيرات الهيكلي?...

Mouse models of heart disease, such as TAC and myocardial infarction (MI), have been proven to be valuable to study disease mechanisms as well as to develop novel therapeutic strategies3. TAC initially induces compensatory hypertrophy, but prolonged pressure overload leads to cardiac dilatation and heart failure4. The tightness of the aortic constriction directly determines the degree of cardiac hypertrophy and its transition to heart failure. Noninvasive and reliable measurement of pressure gradient across the aortic constriction is essential for the success of these studies. Doppler imaging has been used to assess pressure gradient produced by TAC5, which is a noninvasive alternative for catheter-based pressure measurement.
Echocardiography has been widely used to noninvasively measure cardiac morphology as well as systolic and diastolic function in mice6-8. Two-dimensional B-mode imaging is used to detect abnormal movements or structural changes of the heart. One-dimensional M-mode imaging is used for quantification of cardiac dimensions and contractility. Color and PW Doppler imaging has recently been used on rodent ultrasound, which has broad applications for echocardiography, including measurement of flow directionality and velocity, as well as systolic and diastolic performance9. 
Longitudinal real-time monitoring of cardiac function using echocardiography in B-mode, M-mode, color and PW Doppler mode provides comprehensive assessment of cardiac structure and function in mice under physiological and pathological conditions. Here we provide a detailed description of the use of echocardiographic imaging to monitor dynamic cardiac morphological and functional changes in mice following TAC or sham surgery.

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			<td height="41" style="height:41px;width:339px;">Anesthesia equipment</td>
			<td style="width:343px;">Harvard Apparatus, 84 October Hill Road 
			Holliston, MA</td>
			<td style="width:175px;">723015</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">Vevo 2100 Imaging System</td>
			<td style="width:343px;">VisualSonics Inc., 3080 Yonge Street Suite 6100, Box 66, Toronto, Ontario, Canada</td>
			<td style="width:175px;">Vevo 2100</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">Aquasonic ultrasound gel</td>
			<td style="width:343px;">Parker Laboratories, 286 Eldridge Rd, Fairfield, NJ&#160;</td>
			<td style="width:175px;">03-50</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">Isoflurane</td>
			<td style="width:343px;">Piramal Healthcare, Inc, 3950 Schelden Circle 
			Bethlehem, PA&#160;</td>
			<td style="width:175px;">NDC 66794-017-25</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">F/air anesthesia gas filter unit</td>
			<td style="width:343px;">A.M. Bickford, Inc, 12318 Big Tree Rd, Wales Center, NY&#160;</td>
			<td style="width:175px;">80120</td>
			<td></td>
		</tr>
	</tbody>
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assessment of cardiac morphological and functional changes in mouse model of transverse aortic constriction by echocardiographic imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable noninvasive method is essential to assess real-time cardiac morphological and functional changes in animal models of heart disease. Transthoracic echocardiography represents an important tool for noninvasive assessment of cardiac structure and function2. Here we used a high-resolution ultrasound imaging system to monitor myocardial remodeling and heart failure progression over time in a mouse model of TAC. B-mode, M-mode, and Doppler imaging were used to precisely assess cardiac hypertrophy, ventricular dilatation, and functional deterioration in mice following TAC. Color and pulse wave (PW) Doppler imaging was used to noninvasively measure pressure gradient across the aortic constriction created by TAC and to assess transmitral blood flow in mice. Thus transthoracic echocardiographic imaging provides comprehensive noninvasive measurements of cardiac dimensions and function in mouse models of heart disease.

ويبين الشكل 1 صور B-وضع وترى قوس الأبهر من قلب فأر تعرض لخدعة (الشكل 1A) أو جراحة TAC (الشكل 1B). قوس الأبهر، الشريان اللا اسم له، غادر الشريان السباتي المشترك، وتعرض الشريان تحت الترقوة الأيسر. لاحظ أن انقباض الشريان الأورطي وا?...

Watch this Scientific Journal Video about Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging at JoVE.com

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

والهدف من هذا البروتوكول هو تقييم noninvasively التغييرات الهيكلية والوظيفية القلب في نموذج الفأر من أمراض القلب التي أنشأتها انقباض الشريان الأورطي عرضية، وذلك باستخدام B- و M-وضع تخطيط صدى القلب واللون / نبض موجة دوبلر التصوير.

تقييم القلب المورفولوجية والوظيفية التغييرات في الفأر نموذج من مستعرض الأورطي انقباض بواسطة تخطيط صدى القلب التصوير

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Research

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