Name: assessment of cardiac morphological and functional changes in mouse model of transverse aortic constriction by echocardiographic imaging
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Description: Watch this Scientific Journal Video about Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging at JoVE.com

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protokol Washington Üniversitesi Kurumsal Hayvan Bakım ve Kullanım Komitesi kuralları takip eder. 1. Cerrahi Prosedür ve Görüntüleme Hazırlık <ol><li> TAC ya da sham tabi C57BL / 6 fareleri, daha önce 10 tarif. </li><li> Bir hafta TAC veya sahte ameliyat sonrası, 1 L / dk O 2 ile karışık% 2 izofluran ile indüksiyon odasında fare uyutmak. ayak veya kuyruk kısma tepkisizlik tarafından uygun anesthetization onaylayın. anestezi altında iken kuruluğun...

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Ekokardiyografi yaygın kalp hastalığı 2,6 kemirgen modellerinde kardiyak fonksiyonunu değerlendirmek için kullanılır olmuştur. Bu tür basınç-hacim döngü ölçümü 11 gibi invaziv veya terminal yöntemlere kıyasla ve ex vivo kalp 12, ekokardiyografi yaşayan hayvanlarda süregelen kalp yapısal ve fonksiyonel değişikliklerin değerlendirilmesi için güçlü, non-invaziv bir araç sağlar çalışma. Güvenilir veri elde etmek için, dikkatli ısıtma cihazı ve...

Mouse models of heart disease, such as TAC and myocardial infarction (MI), have been proven to be valuable to study disease mechanisms as well as to develop novel therapeutic strategies3. TAC initially induces compensatory hypertrophy, but prolonged pressure overload leads to cardiac dilatation and heart failure4. The tightness of the aortic constriction directly determines the degree of cardiac hypertrophy and its transition to heart failure. Noninvasive and reliable measurement of pressure gradient across the aortic constriction is essential for the success of these studies. Doppler imaging has been used to assess pressure gradient produced by TAC5, which is a noninvasive alternative for catheter-based pressure measurement.
Echocardiography has been widely used to noninvasively measure cardiac morphology as well as systolic and diastolic function in mice6-8. Two-dimensional B-mode imaging is used to detect abnormal movements or structural changes of the heart. One-dimensional M-mode imaging is used for quantification of cardiac dimensions and contractility. Color and PW Doppler imaging has recently been used on rodent ultrasound, which has broad applications for echocardiography, including measurement of flow directionality and velocity, as well as systolic and diastolic performance9. 
Longitudinal real-time monitoring of cardiac function using echocardiography in B-mode, M-mode, color and PW Doppler mode provides comprehensive assessment of cardiac structure and function in mice under physiological and pathological conditions. Here we provide a detailed description of the use of echocardiographic imaging to monitor dynamic cardiac morphological and functional changes in mice following TAC or sham surgery.

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			<td height="41" style="height:41px;width:339px;">Anesthesia equipment</td>
			<td style="width:343px;">Harvard Apparatus, 84 October Hill Road 
			Holliston, MA</td>
			<td style="width:175px;">723015</td>
			<td style="width:167px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">Vevo 2100 Imaging System</td>
			<td style="width:343px;">VisualSonics Inc., 3080 Yonge Street Suite 6100, Box 66, Toronto, Ontario, Canada</td>
			<td style="width:175px;">Vevo 2100</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">Aquasonic ultrasound gel</td>
			<td style="width:343px;">Parker Laboratories, 286 Eldridge Rd, Fairfield, NJ&#160;</td>
			<td style="width:175px;">03-50</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">Isoflurane</td>
			<td style="width:343px;">Piramal Healthcare, Inc, 3950 Schelden Circle 
			Bethlehem, PA&#160;</td>
			<td style="width:175px;">NDC 66794-017-25</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:339px;">F/air anesthesia gas filter unit</td>
			<td style="width:343px;">A.M. Bickford, Inc, 12318 Big Tree Rd, Wales Center, NY&#160;</td>
			<td style="width:175px;">80120</td>
			<td></td>
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assessment of cardiac morphological and functional changes in mouse model of transverse aortic constriction by echocardiographic imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable noninvasive method is essential to assess real-time cardiac morphological and functional changes in animal models of heart disease. Transthoracic echocardiography represents an important tool for noninvasive assessment of cardiac structure and function2. Here we used a high-resolution ultrasound imaging system to monitor myocardial remodeling and heart failure progression over time in a mouse model of TAC. B-mode, M-mode, and Doppler imaging were used to precisely assess cardiac hypertrophy, ventricular dilatation, and functional deterioration in mice following TAC. Color and pulse wave (PW) Doppler imaging was used to noninvasively measure pressure gradient across the aortic constriction created by TAC and to assess transmitral blood flow in mice. Thus transthoracic echocardiographic imaging provides comprehensive noninvasive measurements of cardiac dimensions and function in mouse models of heart disease.

Şekil 1 sahte (Şekil 1A) tabi fare kalp veya TAC cerrahisi (Şekil 1B) aortik ark bakış B-mod görüntüleri gösterir. arkus aorta, innominat arter, karotis arter sol ve sol subklavyen arter gösterilir. Aort daralma TAC&#39;de açıkça görünür olduğunu unutmayın, ancak kalbi sham değil. Aort görünümünden Renkli Doppler görüntüleri Şekil 2A gösterilmiştir. Daralma site genelinde aort akım dalga PW ...

Watch this Scientific Journal Video about Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging at JoVE.com

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Bu protokolün amacı, noninvaziv B- ve M-mod ekokardiyografi ve renk / nabız dalga Doppler görüntüleme kullanarak, enine aort daralma yarattığı kalp hastalığı fare modelinde kardiyak yapısal ve fonksiyonel değişikliklerin değerlendirilmesidir.

Ekokardiyografik Imaging tarafından Enine Aort Konstrüksiyon Mouse Modeli Kardiyak Morfolojik Değerlendirilmesi ve Fonksiyonel Değişiklikler

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

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