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DOI :
10.3791/54112-v
•
11:02 min
May 17th, 2016
Chapters
0:05
Title
1:07
Isolation of Nuclei from Intact Cells
3:18
Extraction and Purification of Histones from Nuclei
5:53
Chemical Derivatization of Histones Using Propionic Anhydride for Bottom-up Analysis
7:43
Sample Desalting with Stage-tips
8:59
Results: Characterization of Histone Post-translational Modifications
10:10
Conclusion
这个协议概述了表征使用质谱(MS)组蛋白的翻译后修饰的完全集成的工作流程。该工作流包括使用纳米流液相色谱法和用于数据分析的指令的细胞培养物或组织中,组蛋白衍生和消化,MS分析组蛋白纯化。该协议被设计为内完成2 - 3天。
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