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Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)

DOI :

10.3791/54113-v

June 20th, 2016

June 20th, 2016

8,124 Views

1Genetics, Development and Cell Biology, Iowa State University, 2Department of Biological Sciences, University of Cincinnati, 3Department of Biology, University of Maryland

Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.

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