June 20th, 2016
•Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.
Related Videos
Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH
Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio) (Video) | JoVE
Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay (Video) | JoVE
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs) (Video) | JoVE
Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation (Video) | JoVE
In Vivo Modeling of the Morbid Human Genome using Danio rerio (Video) | JoVE
Mouse Genome Engineering Using Designer Nucleases (Video) | JoVE
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy (Video) | JoVE
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR (Video) | JoVE
Gamete Collection and In Vitro Fertilization of Astyanax mexicanus (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved