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Mouse Genome Engineering Using Designer Nucleases

DOI :

10.3791/50930-v

12:04 min

April 2nd, 2014

April 2nd, 2014

28,291 Views

1Institute of Laboratory Animal Science, University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of Minnesota

Designer nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be used to modify the genome of mouse preimplantation embryos by triggering both the nonhomologous end joining (NHEJ) and homologous recombination (HR) pathways. These advances enable the rapid generation of mice with precise genetic modifications.

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Mouse Genome Engineering

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