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DOI :
10.3791/54113-v
June 20th, 2016
Chapters
0:05
Title
0:34
mRNA Transcription of Transcription Activator-like Effector Nucleases
2:18
Inject Astyanax mexicanus Embryos with TALEN mRNA
4:06
Phenotype Founder Fish and Evaluate TALEN Efficiency
5:27
Results: Germline Transmission of TALEN-induced oca2 Mutations in A. mexicanus
6:47
Conclusion
遺伝子標的化突然変異誘発は、ゲノム編集の技術を使用して、生物の幅広いことが可能です。ここで、我々はアステュアナクスmexicanus、表面の魚や洞窟魚が含まれて魚の種におけるエフェクターヌクレアーゼ(TALENs)などの転写活性化因子を用いて標的遺伝子の突然変異誘発のためのプロトコルを示しています。
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