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DOI :
10.3791/54115-v
•
11:31 min
June 30th, 2016
Chapters
0:05
Title
0:59
Generation of GFP Tagged c-di-GMP Reporter P. aeruginosa Strain
4:21
Preparation of the Starter Culture for Inoculation
5:35
Inoculation and Incubation of the 384-well Plates Containing Small Molecules
6:33
Measurement and Analysis of Growth and Intracellular c-di-GMP Level
8:25
Results: Hits Obtained Following the High-throughput Screen against P. aeruginosa
10:07
Conclusion
이 문서에서는 성공적으로 항균 약물 발견 및 화합물 테스트를위한 새로운 강력한 도구를 제공, 녹농균에 순환 디 GMP의 세포 수준을 조작 할 수있는 잠재적 능력을 작은 분자의 큰 라이브러리를 스크리닝하기 위해 설립 된 높은 처리량 분석에 대해 설명합니다.
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