0:43
Synthesis of NNS Sub-libraries for Each Amino Acid Position by Two-step PCR Site-directed Mutagenesis
1:53
Cloning of NNS Sub-libraries into Selection Vectors
3:47
Selection of the TEM-1 Whole-Protein Saturation Mutagenesis Library for Antibiotic Resistance
5:51
High Throughput Sequencing to Determine Fitness Effects of Mutations
8:44
Results: High Throughput Sequencing from Whole-Protein Saturation Mutagenesis