Оптимальное Лентивирус производства и клеточной культуры условия, необходимые для успешного трансдукции Первичные человеческие бронхиальные эпителиальные клетки

Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.