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DOI :
10.3791/54203-v
June 29th, 2016
Chapters
0:05
Title
0:48
Chinese Hamster Ovary (CHO)-Intercellular Adhesion Molecule (ICAM) Cell Plating
1:50
T Cell Preparation
2:46
Input/Output Hosing and Syringe Pump Preparation
3:59
Flow Chamber Loading and Cell Stimulation
5:53
Results: SDF-1alpha Induced T Cell Adhesion to ICAM-1 Under Shear Stress
6:51
Conclusion
この流接着アッセイは、T細胞、上皮細胞間相互作用の単純な、耐衝撃性モデルを提供します。シリンジポンプは、剪断応力を生成するために使用し、共焦点顕微鏡は、定量化のための画像を捕捉します。これらの研究の目的は、効果的に流れ条件を使用して、T細胞接着を定量化することです。
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