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DOI :
10.3791/54210-v
July 6th, 2016
Chapters
0:05
Title
1:02
Reverse Transfection of siRNA and Seeding of HeLa 229 Cells
4:25
Infection of Reverse Transfected Cells
5:39
Addition of BlaM Substrate to Determine Level of Translocation
8:23
Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells
9:37
Conclusion
세균성 병원균과 호스트 사이의 상호 작용을 조사하는 것은 생물학적 연구의 중요한 영역입니다. 여기서는 BLAM 기판을 사용하여 siRNA의 유전자 사일런 동안 Coxiella burnetii에 의해 이펙터 전위를 측정하기 위해 필요한 기술들을 설명한다.
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