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DOI :
10.3791/54219-v
July 30th, 2016
Chapters
0:05
Title
0:43
Bacterial Culture and Diet Preparation
2:16
Egg-laying Cages
3:13
Egg Collection
3:55
Egg Dechorionation and Sterile Diet Transfer
5:33
Gnotobiotic Fly Generation
6:40
Measuring CFU Load and Testing for Sterility
8:05
Results: Successful Axenic Fly Rearing Confirmed with CFUs
8:49
Conclusion
提出了一种用于无菌和限菌条件下饲养果蝇的方法。飞胚dechorionated在次氯酸钠,无菌转移到无菌的饮食,并在密闭容器中饲养。接种饮食和胚胎与细菌导致限菌协会,和细菌的存在是通过电镀全身果蝇匀浆证实。
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