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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

DOI :

10.3791/54281-v

December 25th, 2016

December 25th, 2016

13,941 Views

1Department of Pediatrics & Human Development, Institute for Integrative Toxicology, Michigan State University, 2RECETOX — Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University

This protocol describes a scalpel loading-fluorescent dye transfer technique that measures intercellular communication through gap junction channels. Gap junctional intercellular communication is a major cellular process by which tissue homeostasis is maintained and disruption of this cell signaling has adverse health effects.

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