JoVE Logo
Faculty Resource Center

Sign In

Using Unfixed, Frozen Tissues to Study Natural Mucin Distribution

DOI :

10.3791/3928-v

11:39 min

September 21st, 2012

September 21st, 2012

47,021 Views

1Department of Cellular and Molecular Medicine, University of California, San Diego , 2Biosecurity and Public Health, Los Alamos National Laboratory

Unfixed frozen tissue samples embedded in Optimal Cutting Temperature medium (OCT) can be used to study natural distribution and glycosylation of secreted mucus. In this approach tissue processing is minimal and the natural presentation of glycolipids, mucins and glycan-epitopes is preserved. Tissue sections can be analyzed by immunohistochemistry using fluorescence or chromogenic detection.

Tags

Unfixed Tissues

-- Views

Related Videos

article

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

article

Computer-Generated Animal Model Stimuli

article

Using the optokinetic response to study visual function of zebrafish

article

Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

article

High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately

article

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module

article

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

article

At-Risk Butterfly Captive Propagation Programs to Enhance Life History Knowledge and Effective Ex Situ Conservation Techniques

article

Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations

article

A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved