Caracterización de las células dendríticas derivadas de monocitos humanos mediante imágenes de Citometría de Flujo: una comparación entre dos protocolos de aislamiento de monocitos

This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.