Perform ATP Hydrolysis Reaction with Purified Protein
3:09
Incubate Samples Containing Free Pi with Detection Reagent
5:44
Results: Quantitation of In Vitro ATPase Activity
6:59
Conclusion
We describe a basic protocol for quantitating in vitro ATPase activity. This protocol can be optimized based on the level of activity and requirements for a given purified ATPase.