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DOI :
10.3791/54313-v
•
9:38 min
October 16th, 2016
Chapters
0:05
Title
1:03
Collection and Fertilization of Ciona intestinalis Gametes
2:43
Dechorionization of Zygotes
4:44
Electroporation of Zygotes
5:58
Microinjection of Unfertilized Dechorionated Eggs
7:40
Results: Microinjection and Electroporation of Ciona intestinalis Embryos
8:51
Conclusion
我们目前的转基因瞬时和基因敲除在玻璃海鞘 ,脊索动物姐妹群脊椎动物,利用显微注射和电技术。这种方法有利于在这个简单的无脊椎动物,具有脊椎动物中最起码的特征,包括脊索和头部感觉上皮细胞,与人类疾病相关的基因的同源基因的许多功能基因组学。
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