First Fibrin Gel Layer Preparation and Fibrin Gel Generation
4:03
Second Fibrin Gel Layer Preparation and Collagen Plug Sandwiching
5:33
Cell Culture Medium Conditions
6:27
Results: Representative Tumor Cell 3D Culture, Invasiveness, and Satellite Tumor Formation
7:58
Conclusion
Transcript
The overall goal of this procedure is to sandwich collagen embedded cancer cells within a fibrin gel to generate a 3D culture system for the monitoring of satellite tumor invasiveness and formation. Our 3D picture system can offer new avenues for
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Cancer cells are embedded in a collagen gel and then sandwiched in an acellular fibrin gel to generate a 3D culture system in which the invasiveness and formation of satellite tumors may be monitored.