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DOI :
10.3791/54355-v
August 6th, 2016
Chapters
0:05
Title
0:45
Harvest of Mouse Thymus
1:52
Agarose Embedding and Vibratome Sectioning of Thymic Lobes
5:30
Preparation of Thymocytes for Overlaying
6:59
Overlaying Thymocytes onto the Thymic Slices
7:48
Results: Positive and Negative Selection of Thymocytes
9:29
Conclusion
我们描述的是,在用流式细胞仪组合,可以被用来建模显影T细胞的阳性和阴性选择胸腺切片的制备方法。胸腺片也可以适用于胸腺细胞迁移,本地化的原位分析,并通过免疫荧光和双光子显微镜信令。
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