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DOI :
10.3791/54355-v
August 6th, 2016
Chapters
0:05
Title
0:45
Harvest of Mouse Thymus
1:52
Agarose Embedding and Vibratome Sectioning of Thymic Lobes
5:30
Preparation of Thymocytes for Overlaying
6:59
Overlaying Thymocytes onto the Thymic Slices
7:48
Results: Positive and Negative Selection of Thymocytes
9:29
Conclusion
我々は、フローサイトメトリーと組み合わせて、T細胞の開発の正および負の選択をモデル化するために使用することができ、胸腺切片の調製を記載します。胸腺スライスはまた胸腺細胞遊走、局在のその場での分析のために適合され、そして免疫蛍光及び二光子顕微鏡検査を介してシグナル伝達することができます。
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