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DOI :
10.3791/54358-v
August 27th, 2016
Chapters
0:05
Title
0:43
Tissue Collection and Dissection
2:17
Cell Dissociation, Fixation and Permeabilization
5:52
Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
7:16
Conclusion
在这里,我们提出了一个荧光活化细胞分选(FACS)协议来研究分子改建的Fos表达来自新鲜和冷冻的脑组织神经元歌舞团。使用冷冻组织可在多个会话许多脑区的FACS隔离,最大限度地利用宝贵的动物主题。
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