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DOI :
10.3791/54358-v
August 27th, 2016
Chapters
0:05
Title
0:43
Tissue Collection and Dissection
2:17
Cell Dissociation, Fixation and Permeabilization
5:52
Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
7:16
Conclusion
ここでは、生鮮・冷凍の両方の脳組織から神経アンサンブルをフォスが発現に分子変化を研究するための蛍光活性化細胞ソーティング(FACS)プロトコルを提示します。凍結組織の使用は、貴重な動物被験体の使用を最大化するために、複数のセッションに比べて多くの脳領域のFACS単離を可能にします。
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