Name: ultrasound-based pulse wave velocity evaluation in mice
Uploaded: 2017-02-14T13:00:00
Description: Watch this Scientific Journal Video about Ultrasound-based Pulse Wave Velocity Evaluation in Mice at JoVE.com

动物实验均按照欧盟指令（2010/63 / UE）和意法（D.Lvo二千〇一十四分之二十六）进行，它遵循实验室动物护理的原则。本地伦理委员会批准批准了研究。 1.成像过程<ol><li>鼠标放置在1升/分钟的纯氧气填充有2.5％的异氟醚麻醉诱导室中。通过反应迟钝到脚趾捏核实麻醉深度。 </li><li>放置在温度控制板上的动物以仰卧姿势。滋润动物的眼睛眼药，以避免它们干涸。通过将鼠标的鼻子中的专用?...

<ol>
	<li>Zaragoza, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+Models+of+Cardiovascular+Diseases.">Animal Models of Cardiovascular Diseases.</a> J Biomed Biotechnol. 2011, 497-841 (2011).</li><li>Laurent, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expert+consensus+document+on+arterial+stiffness:+methodological+issues+and+clinical+applications.">Expert consensus document on arterial stiffness: methodological issues and clinical applications.</a> Eur Heart J. 27, 2588-2605 (2006).</li><li>Wang, Y. X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+aortic+stiffness+assessed+by+pulse+wave+velocity+in+apolipoprotein+E-deficient+mice.">Increased aortic stiffness assessed by pulse wave velocity in apolipoprotein E-deficient mice.</a> Am. J. Physiol. Heart Circ. Physiol. 278, 428-434 (2000).</li><li>Mitchell, G. F., Pfeffer, M. A., Finn, P. V., Pfeffer, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+techniques+for+measuring+pulse-wave+velocity+in+the+rat.">Comparison of techniques for measuring pulse-wave velocity in the rat.</a> J Appl Physiol. 82 (1), 203-210 (1997).</li><li>Parczyk, M., Herold, V., Klug, G., Bauer, W. R., Rommel, E., Jakob, P. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regional+in+vivo+transit+time+measurements+of+aortic+pulse+wave+velocity+in+mice+with+high-field+CMR+at+17.6+Tesla.">Regional in vivo transit time measurements of aortic pulse wave velocity in mice with high-field CMR at 17.6 Tesla.</a> J Cardiovasc Magn Reson. 12, 72 (2010).</li><li>Hartley, C. J., Taffet, G. E., Michael, L. H., Pham, T. T., Entman, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+determination+of+pulse-wave+velocity+in+mice.">Noninvasive determination of pulse-wave velocity in mice.</a> Am J Physiol. 273 (1), 494-500 (1997).</li><li>Feng, J., Khir, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+wave+speed+and+wave+separation+in+the+arteries+using+diameter+and+velocity.">Determination of wave speed and wave separation in the arteries using diameter and velocity.</a> J Biomech. 43 (3), 455-462 (2010).</li><li>Borlotti, A., Khir, A. W., Rietzschel, E. R., De Buyzere, M. L., Vermeersch, S., Segers, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+determination+of+local+pulse+wave+velocity+and+wave+intensity:+changes+with+age+and+gender+in+the+carotid+and+femoral+arteries+of+healthy+human.">Noninvasive determination of local pulse wave velocity and wave intensity: changes with age and gender in the carotid and femoral arteries of healthy human.</a> J Appl Physiol. 113 (5), 727-735 (2012).</li><li>Ch&#233;rin, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrahigh+frame+rate+retrospective+ultrasound+microimaging+and+blood+flow+visualization+in+mice+in+vivo.">Ultrahigh frame rate retrospective ultrasound microimaging and blood flow visualization in mice in vivo.</a> Ultrasound Med Biol. 32 (5), 683-691 (2006).</li><li>Gemignani, V., Faita, F., Ghiadoni, L., Poggianti, E., Demi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+system+for+real-time+measurement+of+the+brachial+artery+diameter+in+B-mode+ultrasound+images.">A system for real-time measurement of the brachial artery diameter in B-mode ultrasound images.</a> IEEE Trans Med Imaging. 26 (3), 393-404 (2006).</li><li>Di Lascio, N., Stea, F., Kusmic, C., Sicari, R., Faita, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-invasive+assessment+of+pulse+wave+velocity+in+mice+by+means+of+ultrasound+images.">Non-invasive assessment of pulse wave velocity in mice by means of ultrasound images.</a> Atherosclerosis. 237 (1), 31-37 (2014).</li><li>Nichols, W. W., O'Rourke, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> McDonald's Blood Flow in Arteries: Theoretical, Experimental, and Clinical Principles. , 215-358 (1998).</li><li>Williams, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+ultrasonic+measurement+of+regional+and+local+pulse+wave+velocity+in+mice.">Noninvasive ultrasonic measurement of regional and local pulse wave velocity in mice.</a> Ultrasound Med Biol. 33 (9), 1368-1375 (2007).</li><li>Penny, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aortic+wave+intensity+of+ventricular-vascular+interaction+during+incremental+dobutamine+infusion+in+adult+sheep.">Aortic wave intensity of ventricular-vascular interaction during incremental dobutamine infusion in adult sheep.</a> Am J Physiol Heart Circ Physiol. 294, 481-489 (2008).</li><li>Segers, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wave+reflection+leads+to+over-+and+underestimation+of+local+wave+speed+by+the+PU-+and+QA-loop+methods:+theoretical+basis+and+solution+to+the+problem.">Wave reflection leads to over- and underestimation of local wave speed by the PU- and QA-loop methods: theoretical basis and solution to the problem.</a> Physiol Meas. 35 (5), 847-861 (2014).</li></ol>

在这项研究中，基于对小鼠PWV评估LND-V循环的图像处理算法已被详细描述。所提出的方法是基于美国图像的处理之用，因此，可以代表现有技术6中 ，13为动脉硬度的在小鼠模型中评估一个有效的替代。事实上，相反地到其基于获取的动脉内压力信号和所需要的动物被处死侵入性的方法如图6所示 ，这种技术是完全非侵入性的，因此，可...

小鼠模型正日益用于心血管疾病的调查（CVD）和在纵向研究允许疾病发展1的不同阶段的表征中是特别使用。大动脉弹性性质与不同病理状况;从技术角度来看，动脉硬度可以通过测量脉搏波速度（PWV），其表示与该脉波在管道中容器2行进的速度进行评估。由于其临床意义，人们越来越即使是在临床前小动物模型3个测量。 不同的技术可用于在小鼠中评估PWV。侵入性方法是基于使用导管末端压力换能器。 PWV是通过在两个不同的动脉部位采集压力信号和除以两个测量之间的距离s评估由信号4之间的时移ITES。有关这类技术的主要缺点是，它们需要两个测量部位，因此，不能在纵向研究中使用之间的距离的评价动物牺牲。为了克服这一限制，可以根据不同的成像技术的非侵入性的方法，已经被开发。以前的研究已经报道通过将上速度编码磁共振成像数据5和脉冲多普勒信号6的渡越时间的方法得到的小鼠PWV评估。然而，用这些方法得到的PWV值是动脉硬化的一个区域评价。事实上，它代表一个平均值，占不同动脉的尺寸和弹性性能方面。此外，这些种类的评价的所需要的两个测量点之间的距离的评估是错误可能INF源luence最终结果。 PWV可以使用直径速度（LND-V）的环7进行评估。此方法是基于直径和流速值的选定容器中的同时评估。按照这种方法，LND-V族环通过绘制自然对数直径值获得VS平均速度值和PWV通过计算对应于早期收缩阶段所得到的循环的线性部分的斜率估算。关于实际执行这种方法，以前的作品已经报道了其在体外的建立系统7应用程序及其为颈动脉和股PWV人类8评估使用效果。 本研究的主要目的是提供一种图像处理算法的详细说明，提供了在使用Ú小鼠非侵入动脉的PWV测量仅s的图像。所提出的方法允许本地动脉硬度的由两个B模式和脉冲波多普勒（PW多普勒）的图像，并且可以对具有关键的重要性，动脉施加的处理装置的评价如腹主动脉。

<table border="0" cellpadding="0" cellspacing="0" width="772">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">VEVO2100</td>
			<td style="width:260px;">FUJIFILM VisualSonics Inc, Toronto, Canada</td>
			<td style="width:119px;"></td>
			<td style="width:177px;">micro-ultrasound equipment</td>
		</tr>
		<tr height="32">
			<td height="32" style="height:32px;">MS250 Ultrasound Probe</td>
			<td style="width:260px;">FUJIFILM VisualSonics Inc, Toronto, Canada</td>
			<td style="width:119px;"></td>
			<td>micro-ultrasound probe</td>
		</tr>
		<tr height="55">
			<td height="55" style="height:55px;width:216px;">EKV Software</td>
			<td style="width:260px;">FUJIFILM VisualSonics Inc, Toronto, Canada</td>
			<td style="width:119px;"></td>
			<td>Software</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Matlab R2015a&#160;</td>
			<td style="width:260px;">MathWorks Inc, Natick, MA, USA</td>
			<td style="width:119px;"></td>
			<td>Software</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Conductive Paste</td>
			<td style="width:260px;">Chosen by the operator</td>
			<td style="width:119px;"></td>
			<td>Laboratory material</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Petroleum Jelly</td>
			<td style="width:260px;">Chosen by the operator</td>
			<td style="width:119px;"></td>
			<td>Laboratory material</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Depilatory Cream</td>
			<td style="width:260px;">Chosen by the operator</td>
			<td style="width:119px;"></td>
			<td>Laboratory material</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Acoustic Coupling Gel&#160;</td>
			<td style="width:260px;">Chosen by the operator</td>
			<td style="width:119px;"></td>
			<td>Laboratory material</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Developed Matlab Software</td>
			<td style="width:260px;"></td>
			<td style="width:119px;"></td>
			<td>The authors are willing to collaborate with those researchers who are interested in the software and to make the software available under their supervision</td>
		</tr>
	</tbody>
</table>

ultrasound-based pulse wave velocity evaluation in mice

动脉硬度可通过计算脉搏波速度（PWV）， 即 ，与该脉波在管道容器行进的速度进行评价。该参数在它被用于评估在与特定基因型/治疗血管功能的改变或用于表征心血管疾病进展小啮齿动物模型中被越来越多的研究。这个协议描述这导致仅使用超声（US）图像小鼠非侵入动脉的PWV测量的图像处理算法。所提出的技术已被用来评估腹主动脉PWV小鼠，并评估其与年龄相关的变化。 腹主动脉美国扫描是从用装有高频美国探针的特定美国设备气态麻醉下的小鼠获得。 B型和脉搏波的多普勒（PW多普勒）图像，以便分别获得直径和平均血流速度瞬时值，分析。为了这个目的，边缘检测和轮廓跟踪技术使用。单拍平均直径和速度波形是时间对齐和组合，以实现对直径速度（LND-V）的循环。 PWV值从环路，其对应于早期收缩相位的线性部分的斜率获得的。 用本方法，解剖和功能信息有关鼠标腹主动脉可以非侵入性地实现。要求美国图像的处理而已，它可以表示为在小鼠的不同动脉部位的弹性特性方面的非侵入性特征的有用工具。本技术的应用可以很容易地扩展到其他血管区，如颈内动脉，因此提供了可能性，以获得一个多站点动脉僵硬度评估。

所提出的方法已被应用到小鼠腹主动脉在先前的研究11。下图显示了真实影像小鼠所描述的方法的应用程序的结果。这些数据是从一个单一的动物（野生型小鼠13周龄，应变：C57BL6，重量33 g）在特别地， 图1表示美国图像的分析的结果。适用于具有高帧率心电门控模式提供直径波形获得的B型图像边缘检测和轮廓跟踪技术;另一方面，对PW-多普?...

Watch this Scientific Journal Video about Ultrasound-based Pulse Wave Velocity Evaluation in Mice at JoVE.com

Ultrasound-based Pulse Wave Velocity Evaluation in Mice

动脉硬度表示可以被认为是对动脉僵硬度的替代索引在心血管疾病和脉搏波速度（PWV）的关键因素。这个协议描述了用于基于可应用于在不同的动脉部位的超声波图像处理小鼠计算的PWV的图像处理算法。

基于超声脉搏波速度评价小鼠

Arterial stiffness can be evaluated by calculating pulse wave velocity (PWV), i.e., the speed with which the pulse wave travels in a conduit vessel. This ...

Research

JoVE Journal

Medicine

Pulse Wave Velocity Testing in the Baltimore Longitudinal Study of Aging

脉搏波传导速度测试老化的巴尔的摩纵向研究中

Carotid-femoral pulse wave velocity is considered the gold standard for measurements of central arterial stiffness obtained through noninvasive ...

科研

医学

Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

微循环血流速度在大鼠肺转移自动测量

Because the lung is a major target organ of metastatic disease, animal models to study the physiology of pulmonary metastases are of great importance. ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

颈动脉输液的紫杉烷类化合物在小鼠体内的药代动力学和药效学分析

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Ultrasound Based Assessment of Coronary Artery Flow and Coronary Flow Reserve Using the Pressure Overload Model in Mice

冠脉流量和冠状动脉血流储备用小鼠压力超负荷基于模型的超声评估

Transthoracic Doppler echocardiography (TTDE) is a clinically useful, noninvasive tool for studying coronary artery flow velocity and coronary flow ...

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

伤口的牙髓评价一个直接盖髓模型的开发和治疗小鼠中修复性牙本质形成

Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is ...

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

在体内MRI 对小鼠股骨骨愈合过程中骨折愈伤组织发育的评价

Endochondral fracture healing is a complex process involving the development of fibrous, cartilaginous, and osseous tissue in the fracture callus. The ...

Measuring the Carotid to Femoral Pulse Wave Velocity (Cf-PWV) to Evaluate Arterial Stiffness

Measuring the Carotid to Femoral Pulse Wave Velocity Cf-PWV to Evaluate Arterial Stiffness

测量颈动脉股脉搏波速度 (Cf PWV) 评价动脉僵硬度

For the elderly, arterial stiffening is a good marker for aging evaluation and it is recommended that the arterial stiffness be determined noninvasively ...

Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

小鼠肺气肿评价持续压力下的肺固定

Emphysema is a significant feature of chronic obstructive pulmonary disease (COPD). Studies involving an emphysematous mouse model require optimal lung ...

Invasive Hemodynamic Assessment for the Right Ventricular System and Hypoxia-Induced Pulmonary Arterial Hypertension in Mice

小鼠右心室系统及缺氧诱发肺动脉高血压的侵入性血液动力学评估

Pulmonary arterial hypertension (PAH) is a chronic and severe cardiopulmonary disorder. Mice are a popular animal model used to mimic this disease. ...

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Rats are widely used in pharmacokinetics (PK) and toxicokinetic (TK) studies that need to collect a certain amount of blood at specific time points to ...