Name: development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to t cells
Uploaded: 2016-09-18T15:00:00
Description: Watch this Scientific Journal Video about Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells at JoVE.com

JHN is supported by the Swiss National Foundation (SNSF 310030_146290).

小鼠繁殖并保存在乌尔姆大学（德国乌尔姆）的动物设施无特定病原体（SPF）的条件下。所有的动物实验是根据当地的动物使用和保护委员会和国家动物福利法的指导进行。 1. pCFP-OVA质粒的构建<ol><li>扩增使用引物Ova_SpeI_fw（3&#39;- GACCAACTAGTATGGAATTTTGTTTTGATGTATT-5&#39;）和Ova_ClaI_rev（3&#39;- GACCAGATCGATTAAGGGGAAACACATCTGCC-5&#39;）37使用质粒的pCI-OVA（氨苄青霉素抗性）42</...

与所有其他的模式，上述的抗原驱动结肠炎模型可呈现在执行该技术的研究者必须意识到几个问题。当注射OT-II /红+ CD4 + T CD62L +细胞在主机上，研究者必须非常温柔细心针插入腹腔。不这样做可能会导致在小鼠可能导致死亡，或这将不会引起任何疾病的细胞的皮下给药的肠的撕裂。 通常情况下，老鼠会在细胞转移后的第一周体重增加。研究者应当在?...

肠道是暴露于外部环境的人体最大的表面上。居民微生物的广大阵列殖民人体的肠道内形成的肠道菌群（或微生物）。这估计是由最多百万亿微生物细胞和构成生物学1-3知人口最稠密的细菌栖息地之一。在GIT细菌定植肠道的利基在那里生存繁衍4。作为回报，菌群赋予了未编码它的基因组中1个附加的功能特征的主机。例如微生物群刺激上皮细胞的增殖，通过自身产生承载不能生产维生素，调节代谢和防止病原体4-6。鉴于这种有利的关系，一些作者认为人是“超生物体”或“holobionts”是细菌和人类基因7,8的混合。鉴于（人）主机上的微生物的有利影响，肠道免疫系统需要耐受共生微生物，使他们能够管腔存在，而且杀了，从腔侧9-11入侵的病原体。肠道免疫系统已开发机制无害的和潜在有害的管腔微生物来区分;然而这些机制尚未很好地理解12。维护肠道完整性要求严格调节免疫稳态保持宽容和豁免权13之间的平衡。在免疫稳态不平衡有助于肠道疾病的诱导如炎性肠病（IBD）3,14。 有两种主要类型的IBD：克罗恩病（CD）和溃疡性结肠炎（UC）。患者与这些疾病通常患有直肠出血，严重腹泻和腹痛15,16。 IBD的单一原因仍未知的，但遗传因素，环境因素和错调免疫反应的组合可能是疾病发展的15关键事件。 鸡传染性法氏囊病动物模型已经使用了超过50年。在过去几十年中新的IBD模型系统已被开发来测试关于IBD 17,18的发病机制中的各种假说。慢性结肠炎的最佳表征的模型是诱导T细胞稳态19,20的破坏的T细胞转移模型。该模型涉及到从免疫活性小鼠转印幼稚T细胞分化成缺乏T和B细胞（如RAG - / -和SCID小鼠）的主机16,21。疾病在该模型的发展是通过评估腹泻的存在下，体力活动减少和体重的损失为3-10周监测。这就是所谓的消耗综合征16。相比于健康小鼠移植主机的结肠组织是thickeR，短重16。使用T细胞转移模型中，也可以理解的T细胞群如何不同可以向IBD 22的发病机制。 T细胞转移模型不分析抗原特异性方式在疾病过程中APC和T细胞之间的相互作用。它已经表明，骨髓细胞和淋巴样细胞之间的相互作用可能是负责肠道炎症23的发展。尽管IBD的许多方面都得到了澄清，仍然需要清楚地理解，导致疾病发展的初始事件。 它已经表明，在不存在的微生物转移结肠炎不能建立24。最近，一些理论认为IBD可以针对共生细菌25的免疫应答的结果。作者也提出了共生菌是必不可少的诱发炎症在远端肠26，在无菌（GF）的动物的肠道免疫系统通常受损27,28，但这些小鼠中完全胜任肠道免疫系统29的发展无特定病原体动物细菌结果的混合物的定植。因此，该微生物群似乎是在IBD的发病机理的一个关键因素，无论是作为可诱发或防止肠炎症30,31的发展的机制。当前理论认为IBD是微生物不平衡，称为生态失调，遗传倾向的患者32的结果，但是它是目前尚不清楚，如果生态失调是原因或疾病12的结果。考虑到微生物在IBD的发展中的作用， 在体外实验表明，CD4 + T细胞可以通过肠道细菌33,34脉冲式装甲运兵车被激活。 此外，已经表明，从抗原不同共生细菌物种，例如大肠杆菌大肠杆菌，类杆菌，真杆菌和变形杆菌 ，能够激活CD4 + T细胞35。这表明，细菌抗原给T细胞的呈现是重要的，对IBD的发展。为了减少通过在疾病过程中的微生物衍生的多种抗原的复杂性，在大肠杆菌菌株已创建产生该OVA抗原。转移结肠炎诱导OVA特异性T细胞注射到RAG - / -动物的OVA表达E.殖民大肠杆菌 。 这种模型是基于最近的证据表明，CX 3 CR1 +国会议员，在结肠固有层（CLP）36的主要细胞亚群，与CD4 + T细胞转移过程中相互作用肠炎37。国会议员采样为颗粒性抗原肠腔，如细 ​​菌，使用他们的树突36，38,39。以往的研究表明，国会议员也可以占用可溶性抗原，如OVA，引入到肠腔40,41。给出的丰度的CX 3 CR1 +国会议员中电的，可能的是这些细胞可以品尝管腔细菌和与CD4的T细胞相互作用。小鼠共焦成像与大肠杆菌定植OVA特异性CD4 + T细胞移植大肠杆菌 CFP-OVA，表明CX 3 CR1 +国会议员都在抗原驱动结肠炎的发展过程中与OT-II CD4 + T细胞接触。这种模式使肠道装甲运兵车和具体仅在肠腔特定的抗原表达细菌性T细胞的抗原呈递过程的研究。

<table border="0" cellpadding="0" cellspacing="0" width="801">
	<tbody>
		<tr height="23">
			<td height="23" style="height:23px;width:216px;">LB Broth, Miller (Luria-Bertani)</td>
			<td style="width:200px;">Difco</td>
			<td style="width:219px;">244620</td>
			<td style="width:167px;"></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Rotary Shake</td>
			<td style="width:200px;">Reiss Laborbedarf e. K.</td>
			<td style="width:219px;">Model 3020 GFL</td>
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			<td height="21" style="height:21px;width:216px;">2 mm gap couvettes&#160;</td>
			<td style="width:200px;">Peqlab Biotechnologie GmbH</td>
			<td style="width:219px;">71-2020</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Glycerol</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">G5516-100ML</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Gene Pulser Xcell system&#160;</td>
			<td style="width:200px;">BioRad Laboratories GmbH</td>
			<td style="width:219px;">1652660</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">LB Agar, Miller (Luria-Bertani)</td>
			<td style="width:200px;">Difco</td>
			<td style="width:219px;">244510</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ampicillin</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">A9393-5G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">SOC Medium</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td>S1797-100ML</td>
			<td></td>
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		<tr height="16">
			<td height="16" style="height:16px;width:216px;">High Pure Plasmid Isolation Kit</td>
			<td style="width:200px;">Roche</td>
			<td style="width:219px;">11754777001</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Agarose</td>
			<td style="width:200px;">Carl Roth GmbH &#38; Co</td>
			<td style="width:219px;">3810.1</td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:216px;">EDTA</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">E9884-100G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tris-HCl</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">T5941</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Glacial acetic acid</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">537020&#160;</td>
			<td></td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;">Gel chamber&#160;</td>
			<td style="width:200px;">PEQLAB Biotechnology GmbH</td>
			<td style="width:219px;">40-0708</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Loading Dye</td>
			<td style="width:200px;">Thermo Fisher</td>
			<td style="width:219px;">R0611</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">GeneRuler 1 kb DNA Ladder&#160;</td>
			<td style="width:200px;">Thermo Fisher</td>
			<td style="width:219px;">SM0312</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ethidium bromide solution</td>
			<td style="width:200px;">Carl Roth GmbH &#38; Co. KG</td>
			<td style="width:219px;">2218.3</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Photo-documentation system&#160;</td>
			<td style="width:200px;">Decon Science Tech GmbH</td>
			<td style="width:219px;">DeVision G&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">DNA sequencing&#160;</td>
			<td style="width:200px;">MWG-Biotech GmbH</td>
			<td style="width:219px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Phosphate buffered saline (PBS)</td>
			<td style="width:200px;">Biochrom</td>
			<td>L182-50</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Fluorescent microscope&#160;</td>
			<td style="width:200px;">Zeiss</td>
			<td style="width:219px;">HBO 100</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Mini-PROTEAN Tetra System</td>
			<td style="width:200px;">Bio-Rad Laboratories GmbH</td>
			<td style="width:219px;">1658005</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">PageRuler Prestained Protein Ladder&#160;</td>
			<td style="width:200px;">Fermentas, St. Leon-Rot, Germany</td>
			<td style="width:219px;"></td>
			<td></td>
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		<tr height="42">
			<td height="42" style="height:42px;width:216px;">IstanBlue Solution</td>
			<td style="width:200px;">Expedeon, Cambridgeshire, United Kingdom</td>
			<td style="width:219px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Nitrocellulose membrane&#160;</td>
			<td style="width:200px;">Macherey-Nagel GmbH &#38; Co. KG</td>
			<td>741280</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Electro blotter&#160;</td>
			<td style="width:200px;">Biometra GmbH</td>
			<td style="width:219px;">846-015-600</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Bovine Serum Albumins (BSA)</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">A6003-25G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Anti-Ovalbumin antibody&#160;</td>
			<td style="width:200px;">Abcam</td>
			<td style="width:219px;">ab181688</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Anti-rabbit IgG&#160; HRP</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">A0545&#160;</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Pierce ECL Plus Western Blotting Substrate</td>
			<td style="width:200px;">Pierce Biotechnology, Thermo Fischer Scientific Inc</td>
			<td style="width:219px;">32132</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Forene</td>
			<td>Abbott</td>
			<td style="width:219px;">2594.00.00</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">FBS</td>
			<td style="width:200px;">Invitrogen</td>
			<td style="width:219px;">10500-064</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Falcon Cell Strainers</td>
			<td style="width:200px;">Fischer Scientific&#160;</td>
			<td style="width:219px;">08-771-19</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ammonium chloride</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">254134-5G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Tris Base</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">10708976001</td>
			<td></td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;width:216px;">CD4+ CD62 L+ T isolation kit&#160;</td>
			<td style="width:200px;">Miltenyi Biotec</td>
			<td style="width:219px;">130-093-227&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">MACS LS Columns&#160;</td>
			<td style="width:200px;">Miltenyi Biotec</td>
			<td style="width:219px;">130-042-401</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">MACS MS Columns&#160;</td>
			<td style="width:200px;">Miltenyi Biotec</td>
			<td>130-042-201</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">MidiMACS Separator</td>
			<td style="width:200px;">Miltenyi Biotec</td>
			<td style="width:219px;">130-042-302</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">MiniMACS Separator</td>
			<td style="width:200px;">Miltenyi Biotec</td>
			<td style="width:219px;">130-042-102</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">MACS MultiStand</td>
			<td style="width:200px;">Miltenyi Biotec</td>
			<td style="width:219px;">130-042-303</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Feeding Needle 20G</td>
			<td style="width:200px;">SouthPointe Surgical Supply, Inc</td>
			<td style="width:219px;">FN-7903</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Formalin solution, neutral buffered, 10%</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">HT501128</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Paraffin</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">1496904</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hematoxylin</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">H9627</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Eosin Y</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">230251&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Dithiothreitol</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">D9779&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Collagenase type VIII</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">C-2139</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Roswell Park Memorial Institute (RPMI) medium</td>
			<td style="width:200px;">AppliChem</td>
			<td style="width:219px;">A2044, 9050</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Percoll (density 1.124 g/ml)</td>
			<td style="width:200px;">Biochrome</td>
			<td style="width:219px;">L-6145</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sodium azide</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">438456</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Mouse BD Fc Block</td>
			<td style="width:200px;">BD Pharmingen</td>
			<td style="width:219px;">553141</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">FITC-conjugated mAb binding V&#223; 5.1, 5.2&#160;</td>
			<td style="width:200px;">BD Pharmingen</td>
			<td style="width:219px;">553189</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">APC-conjugated mAb binding CD4 GK1.5&#160;</td>
			<td style="width:200px;">eBioscience</td>
			<td style="width:219px;">17-0041-83</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">FACS Calibur&#160;</td>
			<td style="width:200px;">BD Biosciences</td>
			<td style="width:219px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">FCS Express V3 software</td>
			<td style="width:200px;">DeNovo</td>
			<td style="width:219px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Meta scanning confocal microscope&#160;</td>
			<td style="width:200px;">Zeiss</td>
			<td style="width:219px;">LSM 710&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Zeiss Workstation</td>
			<td style="width:200px;">Zeiss</td>
			<td style="width:219px;">LSM 7</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Zeiss ZEM software&#160;</td>
			<td style="width:200px;">Zeiss</td>
			<td style="width:219px;">v4.2.0.121</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Maxisorp immuno plates&#160;</td>
			<td style="width:200px;">NUNC, Roskilde</td>
			<td style="width:219px;">442404</td>
			<td></td>
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		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Streptavidin conjugated alkaline phosphatase</td>
			<td style="width:200px;">Jackson Immuno Research</td>
			<td style="width:219px;">016-050-084</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Alkaline phosphatase substrate 4-Nitrophenyl phosphate disodium salt hexahydrate</td>
			<td style="width:200px;">Sigma-Aldrich</td>
			<td style="width:219px;">71768-5G</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">mAb R4-6A2</td>
			<td style="width:200px;">BD Biosciences</td>
			<td style="width:219px;">551216</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">mAb XMG1.2&#160;</td>
			<td style="width:200px;">BD Biosciences</td>
			<td>554410</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">TECAN microplate-ELISA reader</td>
			<td style="width:200px;">Tecan</td>
			<td style="width:219px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">EasyWin software</td>
			<td style="width:200px;">Tecan</td>
			<td style="width:219px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

development of an antigen-driven colitis model to study presentation of antigens by antigen presenting cells to t cells

Inflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological mechanisms involved during the disease is the T cell transfer model of colitis. In this model, immunodeficient mice (RAG-/- recipients) are reconstituted with naive CD4+ T cells from healthy wild type hosts.
This model allows examination of the earliest immunological events leading to disease and chronic inflammation, when the gut inflammation perpetuates but does not depend on a defined antigen. To study the potential role of antigen presenting cells (APCs) in the disease process, it is helpful to have an antigen-driven disease model, in which a defined commensal-derived antigen leads to colitis. An antigen driven-colitis model has hence been developed. In this model OT-II CD4+ T cells, that can recognize only specific epitopes in the OVA protein, are transferred into RAG-/- hosts challenged with CFP-OVA-expressing E. coli. This model allows the examination of interactions between APCs and T cells in the lamina propria.

要建立抗原驱动结肠炎模型中的E.大肠杆菌菌株 已构建了包含在其中在强组成型启动子P 超 （ 图1A）的控制被表示为在CFP的基因融合到为鸡卵清蛋白和所述融合构建体的编码序列的质粒。荧光显微镜显示重组大肠杆菌大肠杆菌 pCFP-OVA，但不是亲本大肠杆菌大肠杆菌 DH10B，表达CFP（ 图1B）。 CFP-OVA-生产E.?...

Watch this Scientific Journal Video about Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells at JoVE.com

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

抗原驱动结肠炎模型的开发由抗原呈递细胞T细胞抗原研究的介绍

Inflammatory bowel disease (IBD) is a chronic inflammation which affects the gastrointestinal tract (GIT). One of the best ways to study the immunological ...

Research

JoVE Journal

Immunology and Infection

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

HLA - IG基于人工抗原提呈细胞可高效体外扩展人类的CTL

CTL with optimal effector function play critical roles in mediating protection against various intracellular infections and cancer. However, individuals ...

科研

免疫与感染

Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

在细胞命运<em&gt; E.大肠杆菌</em感染后的噬菌体λ

The system comprising bacteriophage (phage) lambda and the bacterium E. coli has long served as a paradigm for cell-fate determination1,2. Following the ...

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Artificial Antigen Presenting Cell aAPC Mediated Activation and Expansion of Natural Killer T Cells

人工抗原提呈细胞（AAPC）的自然杀伤T细胞介导的​​激活和扩展

Natural killer T (NKT) cells are a unique subset of T cells that display markers characteristic of both natural killer (NK) cells and T cells1. Unlike ...

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

临床应用<em&gt;睡美人</em和人工抗原呈递细胞基因修饰T细胞从外设和脐带血中

The potency of clinical-grade T cells can be improved by combining gene therapy with immunotherapy to engineer  a biologic product with the potential for ...

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

腺病毒转导朴素的CD4 T细胞调节性T细胞分化研究

Regulatory T cells (Tregs) are essential to provide immune tolerance to self as well as to certain foreign antigens. Tregs can be generated from naive CD4 ...

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Killer Artificial Antigen Presenting Cells KaAPC for Efficient In Vitro Depletion of Human Antigen-specific T Cells

杀手人工抗原提呈细胞（KaAPC）为有效<em&gt;在体外</em&gt;人抗原特异性T细胞耗竭

Current treatment of T cell mediated autoimmune diseases relies mostly on strategies of global immunosuppression, which, in the long term, is accompanied ...

T Cells Capture Bacteria by Transinfection from Dendritic Cells

性T细胞Transinfection从树突状细胞捕捉细菌

Recently, we have shown, contrary to what is described, that CD4+ T cells, the paradigm of adaptive immune cells, capture bacteria from infected dendritic ...

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity

干细胞衍生的抗原特异性调节性T细胞对自身免疫的发展

Autoimmune diseases arise due to the loss of immunological self-tolerance. Regulatory T cells (Tregs) are important mediators of immunologic ...

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-&#945;

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-ÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Â±

人干扰素α超灵敏单分子阵列数字酶联免疫吸附试验的研制与验证

The main aim of this protocol is to describe the development and validation of an interferon (IFN)-&#945; single molecule array digital Enzyme-Linked ...

An In Vivo Mouse Model to Measure Na&#239;ve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

An In Vivo Mouse Model to Measure NaÃƒÆ’Ã†'Ãƒâ€šÃ‚Â¯ve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1 分化的体外模型研究

Quantification of na&#239;ve CD4 T cell activation, proliferation, and differentiation to T helper 1 (Th1) cells is a useful way to assess the role played ...