Name: assays to detect uv-reflecting structures and determine their importance in mate preference using the sailfin molly poecilia latipinna
Uploaded: 2016-09-14T12:30:00
Description: Watch this Scientific Journal Video about Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna at JoVE.com

We are grateful to anonymous reviewers for comments and suggestions that greatly improved this manuscript. We thank R. Bowes, R. Carreno, and T. Panhuis for assistance in collecting the fish. We also thank M. Lee for assistance with male preference trials. We are grateful to the Ohio Wesleyan University Department of Zoology for helpful advice and suggestions throughout this study and Arizona State University (McGraw Lab) for software advice.

所有实验均与俄亥俄卫斯理大学的机构动物护理和使用委员会的批准进行的。 用分光光度计鱼1.记录反射紫外光<ol><li>校准分光光度计和光源与已知白色标准以上的波长范围，以根据仪器或软件的建议来测定。 </li><li>通过在3-氨基苯甲酸乙酯甲磺酸盐的0.5％溶液放置麻醉鱼（MS-222）的缓冲用碳酸氢钠的等量直到鱼变得无法响应。一旦反应迟钝，立即将鱼放在一个黑色，无反射的背景。注：MS...

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分光光度法成功地确定对P. UV标志latipinna。 P的两性latipinna具有沿其两侧UV标记。此外，一些男性对他们背鳍紫外标记，先前发现的性状是在雌性交配偏好7重要。 我们建议使用紫外分光光度法作为一种机制来检测紫外线标志的存在。进一步的测试可能确定其在社会互动，包括交配偏好（如这里详细介绍）的作用。可替代地，紫外线特性可能?...

了解线索，并在动物社会交往使用的信号可以让我们内部和物种之间理解的表型变异。此变化起着进化过程如人口发散，性选择，和形态的重要作用。通常，然而，研究人员正在限于探索最明显的人类感觉系统，特别是那些在视觉或听觉领域内的提示。光度法的使用，然而，使我们能够扩展超出人类可见光谱并进入波长可能在其他物种的社会互动重要我们的调查。 特别是，通过紫外线带来的短距离通信（UV; 300 - 400纳米）的灵敏度必须是择偶1中非常有利的可能性。鸟类和鱼类的许多视觉捕猎的食肉动物，比如，无法检测紫外线辐射。在男性精心显示系统到女性，这些男性会减少他们的捕食的风险，同时保持其通过利用UV光谱，而不是开发线索可检测在可见光谱2,3到吸引异性能力。如果一个发生故障考虑到生物体与每个通信的可能性其他使用这些“私人沟通渠道”，行为和进化显著驱动程序可能会被错过。 该协议概述了调查的茉莉花鳉， 孔雀鱼latipinna，已经没有先前已知的检测UV或利用UV标记能力的一夫多妻鱼使用UV线索的择偶。该鱼种有着密切的亲缘接近其它紫外线敏感的活轴承鱼4和有显微光谱证据表明P. latipinna，与其它茉莉物种如铜绿沿墨西哥和P.台塑 ，拥有一流的视锥细胞（负责统筹感光细胞LOR视觉）是最敏感的UV波长5。在这种性别二态性的物种，女性选择了在男性&#39;颜色鲜艳和扩大散热片6-9的进化中发挥了强有力的作用。这种方法使我们能够探索紫外线是由女性评估男性的质量的附加网上平台。 检测和P.紫外标记测量latipinna使用分光光度计用光纤探头这里详细描述。此外，接受女性mollies是否差异通过光学滤镜观察男性发射全光谱光源包括UV-A关联（[UV +]; 320 - 700纳米），并通过UV阻隔滤镜观察男性（[紫外线]; 400 - 700纳米）进行了讨论。该方法具有用于发现在鱼类和其他生物的UV敏感性和彩色图案，允许研究多种方法包括紫外线和其行为的作用的问题广泛的应用。

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			<td height="21" style="height:21px;width:357px;">Spectrophotometer, P1000</td>
			<td style="width:217px;">Ocean Optics</td>
			<td style="width:119px;"></td>
			<td style="width:351px;">newer models are availabe</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:357px;">DT 1000 xenon UV light source</td>
			<td style="width:217px;">Ocean Optics</td>
			<td style="width:119px;"></td>
			<td>newer models are availabe</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Ocean Optics Overture Software</td>
			<td style="width:217px;">Ocean Optics</td>
			<td style="width:119px;"></td>
			<td>newer software is available</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">R200-Angle-UV bifurcated fiber-optic probe (Guided Wave)</td>
			<td style="width:217px;">Ocean Optics</td>
			<td style="width:119px;"></td>
			<td>newer models are availabe</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Certified reflectance standard, white</td>
			<td style="width:217px;">labsphere</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:357px;">75.7 L Aquarium, divided</td>
			<td style="width:217px;">Experimental Builder</td>
			<td style="width:119px;"></td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:357px;">Full Spectrum Bulb</td>
			<td style="width:217px;">Nature&#39;s Sunlight</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:357px;">UV blocking sheet</td>
			<td style="width:217px;">GAM UV Sheet</td>
			<td style="width:119px;"></td>
			<td></td>
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assays to detect uv-reflecting structures and determine their importance in mate preference using the sailfin molly poecilia latipinna

许多生物过程中的社会交往使用暗示和信号超出人类的敏感性。试图了解他们的行为和生态环境时，要考虑到生物体是如何看待他们的世界是很重要的。灵敏度的紫外光谱（UV; 300 - 400 nm）的跨鸟类，鱼类，爬行类，两栖类，甚至哺乳动物的多个属找到。这个协议描述用于检查生物体紫外线反射结构的存在和用于测试这些线索是否被用作在择偶的上下文社会信号的方法的技术。分光光度计被用来检测反射紫外光和变异在个体和两性之间的反射强度的存在。提出这种技术的一个例子，其中一个二分法的择偶测试暴露性接受个人异性个体，其外观可以通过过滤器，无论是传输全谱或阻挡紫外线的波长进行操作。这个系统允许的女性，而不是男性，sailfin mollies（孔雀鱼latipinna）采用UV的标记，作为其交配的决定的一部分的决心。这些类型的研究，有助于扩大我们的，利用UV生物体的各种知识和洞察UV如何发挥他们生活中的作用。

<html> 图1和2示出了交配偏好水族馆建立和UV测量部位为我们的实验。 测量允许的决心，紫外线反射P. latipinna做具有紫外线的特性，特别是沿其身体（ 图3）的侧面，除了在这些性状的个体差异。一旦UV性状发现，测试显示，女性，而不是男性，P. latipinna在它们的交配决定使用?...

Watch this Scientific Journal Video about Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna at JoVE.com

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

这个协议概括了使用分光光度法来检测生物体紫外线反射结构（在本例中，茉莉花鳉孔雀latipinna），并描述了对分选择测试鱼类允许要在紫外线线索的伴侣选择期间的作用作出推论。

试验以检测紫外线反射结构，并使用茉莉花鳉确定其重要性伴侣偏好<em&gt;孔雀鱼latipinna</em

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Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

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