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DOI :
10.3791/54456-v
•
10:32 min
October 4th, 2016
Chapters
0:05
Title
0:50
Culture of Clonal Sample Neurospheres
2:07
Preparation of Neurosphere-conditioned Medium
4:20
Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
6:25
Differentiation of Sample Neurospheres
7:18
Fixing the Differentiated Neurospheres
8:16
Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
9:28
Conclusion
神経幹細胞(NSCは)自己再生および3神経系統に分化することができる細胞を指します。ここでは、クローン性条件下でニューロスフェア形成および分化を使用して、指定された細胞集団におけるNSCの周波数を決定するためのプロトコルを説明します。
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