Name: micropatterning and assembly of 3d microvessels
Uploaded: 2016-09-09T15:00:00
Description: Watch this Scientific Journal Video about Micropatterning and Assembly of 3D Microvessels at JoVE.com

Forfatterne vil gerne anerkende Lynn og Mike Garvey Imaging Laboratory ved Institut for Stamcelleforskning og regenerativ medicin samt Washington nanofabrikation Facility på University of Washington. De erkender også økonomisk støtte fra National Institute of Health giver DP2DK102258 (til YZ), og uddannelse giver T32EB001650 (til SSK og MAR) og T32HL007312 (til MAR).

1. mikrofabrikation af Mønstret Polydimethylsiloxan (PDMS) med Network Design <ol><li> Wafer Fabrication oprettes en negativ skabelon af netværket Design <ol><li> Opret et netværk mønster ved hjælp af en computer-aided design (CAD) software. Sørg for, at den diagonale dimension mellem ind- og udløb matche afstanden mellem indløbs- og ud- reservoirer på huset enheder i fremtidige skridt (se 2.1.1). Bemærk: Udformningen af det mønster, selv er custom afhængigt af de specifikke forskni...

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Manipulerede mikrokar er en in vitro model, hvor fysiologiske karakteristika såsom luminal geometri, hydrodynamiske kræfter, og multi-cellulære interaktioner er til stede og afstemmelige. Denne type platform er stærk, idet den giver mulighed for at modellere og studere endothelial adfærd i mange forskellige sammenhænge, ​​hvor in vitro-dyrkningsbetingelser kan matches til den for mikromiljøet pågældende. For eksempel de mekanismer køre endoteliale processer, såsom angiogenese, vides at f...

Mikrovaskulaturen i hvert organ til at definere vævet mikromiljø, vedligeholde vævshomeostase og regulere inflammation, permeabilitet, trombose og fibrinolyse 1,2. Mikrovaskulær endotel, især er grænsefladen mellem blodgennemstrømningen og det omgivende væv og derfor spiller en kritisk rolle i modulering vaskulære og organfunktion som reaktion på stimuli, såsom hydrodynamiske kræfter og cirkulerende cytokiner og hormoner 3 - 5. Forståelse af de detaljerede interaktioner mellem endotelet, blod og det omgivende væv mikromiljø er vigtig for studiet af vaskulær biologi og sygdomsprogression. Men fremskridt i at studere disse interaktioner er blevet hæmmet ved begrænset in vitro værktøjer, der kun gentages i vivo mikrovaskulær struktur og funktion 6,7. Som et resultat, har feltet og terapeutiske avancement var stærkt afhængig af dyre og tids-forbrugende dyremodeller, der ofte ikke oversætte til succes i mennesker 8 - 10. Mens in vivo modeller er uvurderlig i studiet af sygdomsmekanismer og vaskulære funktioner, de er komplekse og ofte mangler præcis styring af individuelle cellulære, biokemiske og biofysiske signaler. Kar i hele kroppen besidder en moden hierarkisk struktur i forbindelse med ekspansiv kapillar senge, der giver optimal perfusion og stoftransport samtidigt 11. Oprindeligt vaskulatur former som en primitiv plexus, som reorganiserer til en hierarkisk forgrenet netværk under tidlig udvikling 12,13. Selv om mange af de involverede i disse processer signaler godt forstået 14-16, er det stadig undvigende, hvordan en sådan vaskulær mønster bestemmes 15. Til gengæld den gentog denne proces in vitro til ingeniør organiserede vaskulære netværk har bin vanskelige. Mange eksisterende in vitro platforme til at modellere vaskulatur, såsom todimensionale endotheliale cellekulturer, mangler vigtige egenskaber såsom multi-cellulære nærhed, tredimensionelle luminal geometri, flow, og ekstracellulære matrix. Tube dannelse analyser i 3D hydrogeler (kollagen eller fibrin) 17 - 19 eller invasion assays 20,21 er blevet brugt til at studere endotel funktion i 3D og deres samspil med andre vaskulære 17,22 eller væv celletyper 23. Men samlet lumen i disse analyser mangler sammenkobling, hæmodynamisk flow, og passende perfusion. Endvidere tilbøjeligheden for vaskulær regression i disse kanaldannelse assays 24 forhindrer langtidsdyrkning og modning hvilket begrænser graden af funktionelle undersøgelser, der kan udføres. Således er der et spirende behov for at konstruere in vitro platforme af mikrovaskulære netværk, der passende kan modellere dadothelial egenskaber og er i stand til langtidsdyrkning. En række af vaskulære teknikker er opstået i årenes løb til medicinske anvendelser til at erstatte eller bypass påvirket fartøjer i patienter med vaskulær sygdom. Stor diameter fartøjer fremstillet af syntetiske materialer, såsom polyethylenterephthalat (PET), og polytetrafluorethylen (ePTFE) har haft en betydelig terapeutisk succes med langsigtede åbenhed (gennemsnit 95% åbenhed over 5 år) 25. Selv om små diameter syntetiske transplantater (&lt;6 mm) typisk står komplikationer såsom intimahyperplasi og trombopoiese 26 - 28, manipuleret væv lille diameter transplantater lavet med biologisk materiale har gjort betydelige fremskridt 29,30. Trods fremskridt af denne art, har manipuleret fartøjer på mikroskala forblevet en udfordring. For på tilfredsstillende vis at modellere mikrovaskulaturen, er det nødvendigt at generere komplekse netværk mønstre med til-strækkelig mekanisk styrke til at opretholde åbenheden og med en matrix sammensætning, som giver mulighed for både næringsstoffer permeation for parenchymale celler og cellulær remodeling. Denne protokol udgør en roman kunstigt perfusable fartøj netværk, der efterligner en indfødt in vivo omgivelser med en justerbar og kontrollerbar mikromiljø 31-34. Den beskrevne metode genererer manipuleret mikrokar med diametre i størrelsesordenen 100 um. Manipulerede mikrokar er fremstillet ved perfusion endotelceller via en mikrofluid kanal, der er indlejret i blød type I collagen hydrogel. Dette system har kapacitet til at generere mønstrede netværk med åben luminal struktur, replikere multi-cellulære interaktioner, modulere ekstracellulær matrix sammensætning, og anvende fysiologisk relevante hæmodynamiske kræfter.

<table border="0" cellpadding="0" cellspacing="0" width="1363">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:459px;">Wafer Fabrication</td>
			<td style="width:225px;"></td>
			<td style="width:140px;"></td>
			<td style="width:540px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">AutoGlow Plasma System</td>
			<td>AutoGlow</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Headway Spin Coater</td>
			<td>Headway Research, Inc&#160;</td>
			<td colspan="2">PWM32 Spin Coater&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">ABM Contact Aligner</td>
			<td>AB-M</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Alpha Step Profilometer</td>
			<td>Tencor</td>
			<td>Alpha Step 200</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">SU-8 Developer</td>
			<td>Microchem</td>
			<td>Y020100</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">SU-8 Resist</td>
			<td>Microchem</td>
			<td>SU-8 2000</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">8&#34; silicon wafer</td>
			<td>Wafer World Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tabletop Micro Pattern Generator</td>
			<td>Heidelberg Instruments</td>
			<td>&#956;PG 101</td>
			<td>For generation of photomask</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hot plate</td>
			<td>VWR</td>
			<td>97042-646</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ispropyl alcohol</td>
			<td>Avantor Performance Materials</td>
			<td>9088</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Petri dishes (120 x 120 mm, square)</td>
			<td>Sigma-Aldrich</td>
			<td>Z617679</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trichloro(3,3,3-trifluoropropyl)silane</td>
			<td>Sigma-Aldrich</td>
			<td>MKBG3805V</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Polydimethylsiloxane (PDMS) elastomer base and curing agent</td>
			<td>Dow Corning</td>
			<td>Sylgard 184</td>
			<td>Mixed at 10:1 (w/w)</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Vacuum desiccator</td>
			<td>Sigma-Aldrich</td>
			<td>Z119024-1EA</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Oven</td>
			<td>VWR</td>
			<td>9120976</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Device Fabrication and Culture</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">poly(methyl methacrylate) (PMMA)</td>
			<td>Plexiglas</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Corona Treater</td>
			<td>Electro-Technic Products, Inc.</td>
			<td>BD-20</td>
			<td>Handheld device for plasma treatment of PMMA devices and PDMS molds</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Soldering Iron</td>
			<td>Weller&#160;</td>
			<td>WTCPS</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stainless Steel Truss Head Slotted Machine Screw</td>
			<td>McMaster-Carr&#160;</td>
			<td>91785A096</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stainless steel dowel pins</td>
			<td>McMaster-Carr&#160;</td>
			<td>93600A060</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tweezers&#160;</td>
			<td>Miltex</td>
			<td>24-572</td>
			<td>Any similar tweezers may be used</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Spatula (Micro Spoon)</td>
			<td>Electron Microscopy Services</td>
			<td>62410-01</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Screw driver</td>
			<td></td>
			<td></td>
			<td>Any flat head screwdriver may be used, autoclaved</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Glass coverslips (22 x 22 mm)</td>
			<td>Fisher Scientific</td>
			<td>12-542B</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bleach</td>
			<td>Clorox</td>
			<td>4460030966</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Petri dishes (150 X 25mm)</td>
			<td>Corning</td>
			<td>430599</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Petri dishes (100 X 20 mm)</td>
			<td>Corning</td>
			<td>2909</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cotton, cut into 1 cm x 3 cm pieces</td>
			<td></td>
			<td></td>
			<td>Autoclaved</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Polyethyleneimine (PEI)</td>
			<td>Sigma-Aldrich</td>
			<td>P3143</td>
			<td>Dilute to 1% in cell culture grade water</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Glutaraldehyde</td>
			<td>Sigma-Aldrich</td>
			<td>G6257</td>
			<td>Dilute to 0.1% in cell culture grade water</td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;">Sterile H2O</td>
			<td></td>
			<td></td>
			<td>Autoclaved DI H2O</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Type I collagen, dissolved in 0.1% acetic acid</td>
			<td></td>
			<td></td>
			<td>Isolated from rat tails as described in Rajan et. al. 2006 (ref #37)</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">1 mL syringe</td>
			<td>BD</td>
			<td>309659</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">10 mL syringe</td>
			<td>BD</td>
			<td>309604</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">15 mL conical tubes</td>
			<td>Corning</td>
			<td>352097</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">30 mL conical tubes</td>
			<td>Corning</td>
			<td>352098</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">M199 10X Media&#160;</td>
			<td>Life Technologies</td>
			<td>11825-015</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">1N NaOH (sterile)</td>
			<td>Sigma-Aldrich</td>
			<td>415413</td>
			<td>Dilute to 1N in cell culture grade water</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">HUVECs&#160;</td>
			<td>Lonza</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Endothelial growth media</td>
			<td>Lonza</td>
			<td>CC-3124</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trypsin</td>
			<td>Corning</td>
			<td>25-052-CI</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal bovine serum (FBS)</td>
			<td>Thermofisher Scientific</td>
			<td>10082147</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dextran from Leuconostoc spp. (70kDa)</td>
			<td>Sigma-Aldrich</td>
			<td>31390</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Phosphate Buffered Saline (PBS)</td>
			<td>Corning</td>
			<td>21-031-CV</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hemocytometer</td>
			<td>Hausser Scientific Co.</td>
			<td>3200</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Gel loading tips</td>
			<td>VWR</td>
			<td>37001-152</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">18G Blunt Fill Needle</td>
			<td>BD&#160;</td>
			<td>305180</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">20G Stainless Steel Dispensing Needle</td>
			<td>McMaster-Carr</td>
			<td>75165A123</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tygon 1/32&#8221; ID, 3/32&#34; OD Silicon Tubing</td>
			<td>Cole-Parmer</td>
			<td>EW-95702-00</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">1/16&#34; Tube-to-tube Coupling</td>
			<td>McMaster-Carr</td>
			<td>5116K165</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">90&#176; Elbow Connectors, Tube-to-Tube</td>
			<td>McMaster-Carr</td>
			<td>5121K901</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Luer Lock Coupling (Female, 1/16&#34; ID)</td>
			<td>McMaster-Carr</td>
			<td>51525K211</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Plastic Forceps, with Jaw Grips</td>
			<td>Electron Microscopy Services</td>
			<td>72971</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dual Syringe Pump</td>
			<td>Harvard Apparatus</td>
			<td>70-4505</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">5 mL Polystyrene Round-bottom tube</td>
			<td>Fisher Scientific</td>
			<td>14-959-2A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Device Analysis</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Formaldehyde</td>
			<td>Sigma-Aldrich</td>
			<td>F8775</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bovine Serum Albumin</td>
			<td>Sigma-Aldrich</td>
			<td>A8806-5G</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Triton X-100</td>
			<td>Sigma-Aldrich</td>
			<td>T-9284</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Rabbit anti-hCD31</td>
			<td>Abcam</td>
			<td>ab32457</td>
			<td>1:25 working dilution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">FITC conjugated anti-von Willebrand Factor antibody</td>
			<td>Abcam</td>
			<td>ab8822</td>
			<td>1:100 working dilution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Goat anti-rabbit 568 secondary antibody</td>
			<td>Thermofisher Scientific</td>
			<td>A-11011</td>
			<td>1:100 working dilution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hoescht</td>
			<td>Thermofisher Scientific</td>
			<td>H1399</td>
			<td>Resuspended in DMSO</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium cacodylate&#160;</td>
			<td>Sigma-Aldrich</td>
			<td>C0250</td>
			<td>To make 0.2M cacodylate buffer</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ethanol</td>
			<td>VWR International</td>
			<td>BDH1164-4LP</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">40kDa FITC-conjugated Dextran</td>
			<td>Sigma-Aldrich</td>
			<td>FD40S&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Additional Culture Reagents&#160;</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">CHIR-99021</td>
			<td>Selleck Chem</td>
			<td>S2924</td>
			<td>Small molecule GSK-3 inhibitor</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Human recombinant VEGF</td>
			<td>Peprotech</td>
			<td>100-20</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Human recombinant bFGF</td>
			<td>Peprotech</td>
			<td>AF-100-18B</td>
			<td></td>
		</tr>
	</tbody>
</table>

micropatterning and assembly of 3d microvessels

In vitro platforme til at studere endotelceller og vaskulære biologi er stort set begrænset til 2D endotel cellekultur, flow kamre med polymer eller glas substrater, og hydrogel-baserede rørdannelse assays. Disse analyser, mens informative, ikke rekapitulere lumen geometri, ordentlig ekstracellulære matrix, og multi-cellulære nærhed, som spiller en afgørende rolle i modulerende vaskulær funktion. Dette håndskrift beskriver en sprøjtestøbeproces metode til at generere manipuleret fartøjer med diametre i størrelsesordenen 100 um. Mikrokar er fremstillet ved podning endotelceller i en mikrofluid kanal indlejret i en nativ type I collagen hydrogel. Ved at inkorporere parenchymale celler i collagenmatrix før kanaldannelse kan specifikke væv mikromiljøer modelleres og undersøgt. Yderligere modulationer af hydrodynamisk egenskaber og medier sammensætning muliggør styring af komplekse vaskulær funktion inden for det ønskede mikromiljø.Denne platform muliggør studiet af perivaskulær celle rekruttering, blod-endotel-interaktioner, flow respons, og vævs-mikrovaskulære interaktioner. Udviklet mikrokar tilbyder muligheden for at isolere påvirkningen fra enkelte komponenter i en vaskulær niche og præcist kontrollere sine kemiske, mekaniske og biologiske egenskaber til at studere vaskulær biologi i både sundhed og sygdom.

Den konstruerede beholder platform skaber funktionelle mikrovaskulatur indlejret i en naturlig collagen type I matrix og giver mulighed for stram styring af det cellulære, biofysiske og biokemiske miljø in vitro. For at fabrikere manipuleret mikrokar, er humane navleveneendotelceller (HUVEC&#39;er) perfunderet gennem collagen-embedded mikrofluid netværk, hvor de tillægger danne et patent lumen og sammenflydende endotel. Som illustreret i figur 1A-C, kan fart...

Watch this Scientific Journal Video about Micropatterning and Assembly of 3D Microvessels at JoVE.com

Micropatterning and Assembly of 3D Microvessels

Dette håndskrift præsenterer en sprøjtestøbning metode til at konstruere mikrokar der rekapitule- fysiologiske egenskaber endotel. Den mikro-baseret proces skaber patent 3D vaskulære netværk med skræddersys betingelser, såsom strømning, cellulær sammensætning, form og biokemiske gradienter. Fabrikationsprocessen og eksempler på potentielle anvendelser er beskrevet.

Micropatterning og Samling af 3D mikrokar

In vitro platforms to study endothelial cells and vascular biology are largely limited to 2D endothelial cell culture, flow chambers with polymer or glass ...

Research

JoVE Journal

Bioengineering

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Udarbejdelse af 3D Kollagen Gels og mikrokanalerne for Studiet af 3D Interactions<em&gt; In vivo</em

Historically, most cellular processes have been studied in only 2 dimensions.  While these studies have been informative about general cell signaling ...

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automatiseret robot flydende håndtering forsamling af modulære DNA enheder

Recent advances in modular DNA assembly techniques have enabled synthetic biologists to test significantly more of the available &#34;design space&#34; ...

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Syntese af kationiseret Magnetoferritin for Ultrahurtig magnetisering Celler

Many important biomedical applications, such as cell imaging and remote manipulation, can be achieved by labeling cells with superparamagnetic iron oxide ...

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Bioprinting af brusk og huden væv analoger udnytter en roman passiv blanding enhed teknik for Bioink Precellularization

Bioprinting is a powerful technique for the rapid and reproducible fabrication of constructs for tissue engineering applications. In this study, both ...

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Fabrikation af brugerdefinerede Agarosen Wells for celle såning og væv Ring samlesæt bruger 3D-trykt forme

Engineered tissues are being used clinically for tissue repair and replacement, and are being developed as tools for drug screening and human disease ...

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Protokoller af 3D Bioprinting af gelatine Methacryloyl hydrogel baserede Bioinks

Gelatin methacryloyl (GelMA) has become a popular biomaterial in the field of bioprinting. The derivation of this material is gelatin, which is hydrolyzed ...

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Temperaturstyret samling og karakterisering af en dråbe interface Bilayer

The droplet interface bilayer (DIB) method for assembling lipid bilayers (i.e., DIBs) between lipid-coated aqueous droplets in oil offers key benefits ...

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Kontrol af cellegeometri gennem infrarød laserassisteret mikromaling

Micropatterning is an established technique in the cell biology community used to study connections between the morphology and function of cellular ...

A Micropatterning Assay for Measuring Cell Chirality

Et mikromønsteranalyse til måling af cellechiralitet

Chirality is an intrinsic cellular property, which depicts the asymmetry in terms of polarization along the left-right axis of the cell. As this unique ...

Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting

Gelatine methacryloyl granulære hydrogelstilladser: Mikrogelfabrikation med høj kapacitet, frysetørring, kemisk samling og 3D-bioprint

The emergence of granular hydrogel scaffolds (GHS), fabricated via assembling hydrogel microparticles (HMPs), has enabled microporous scaffold formation ...