Name: micropatterning and assembly of 3d microvessels
Uploaded: 2016-09-09T15:00:00
Description: Watch this Scientific Journal Video about Micropatterning and Assembly of 3D Microvessels at JoVE.com

Forfatterne ønsker å erkjenne Lynn og Mike Garvey Imaging Laboratory ved Institutt for Stem Cell og regenerativ medisin samt Washington Nanofabrication Facility ved University of Washington. De erkjenner også økonomisk støtte fra National Institute of Health gir DP2DK102258 (til YZ), og trening gir T32EB001650 (til SSK og MAR) og T32HL007312 (til MAR).

1. microfabrication av Mønstret Polydimethylsiloxane (PDMS) med Network Design <ol><li> Wafer Fabrication å skape en negativ mal av Network Design <ol><li> Opprett et nettverk mønster ved hjelp av en dataassistert konstruksjon (DAK) programvare. Sørg for at den diagonale dimensjonen mellom innløp og utløp matche avstanden mellom innløps- og utløps reservoarer på huset enheter i fremtidige trinn (se 2.1.1). Merk: Utformingen av mønsteret i seg selv er tilpasset avhengig av de konkrete ...

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Konstruert microvessels er en in vitro modell der fysiologiske egenskaper som luminal geometri, hydrodynamiske krefter, og flercellede interaksjoner er til stede og fleksibel. Denne type plattform er kraftig ved at den gir mulighet til å modellere og studere endothelial atferd i en rekke sammenhenger der vitro dyrkningsforholdene i kan matches til at av mikromiljøet i spørsmålet. For eksempel, de mekanismer som driver endoteliale prosesser, slik som angiogenese, er kjent for å forekomme p...

Microvasculature i hvert organ bidrar til å definere vevet mikromiljøet, vedlikeholde vev homeostase og regulerer betennelse, permeabilitet, trombose, og fibrinolyse 1,2. Mikrovaskulær endotel, i særdeleshet, er grensesnittet mellom blodstrømmen og det omgivende vev, og derfor spiller en avgjørende rolle i modulering av vaskulær og organfunksjon som reaksjon på stimuli slik som hydrodynamiske krefter og sirkulerende cytokiner og hormoner 3 - 5. Å forstå den detaljerte vekselvirkninger mellom endotelet, blod, og det omkringliggende vevet mikro er viktig for studiet av vaskulær biologi og sykdomsprogresjon. Men fremgang i å studere disse interaksjoner har blitt hindret av begrenset in vitro-verktøy som ikke rekapitulere in vivo mikrovaskulær struktur og funksjon 6,7. Som et resultat, har feltet og terapeutiske fremskritt støttet seg tungt på kostbare og tidskonsumere dyremodeller som ofte ikke klarer å oversette til suksess i mennesker 8 - 10. Selv om in vivo-modeller er uvurderlig i studiet av sykdomsmekanismer og vaskulære funksjoner, de er komplekse og ofte mangler nøyaktig kontroll av individuelle celle, biokjemiske og biofysiske signaler. Blodkar i hele kroppen besitter en moden hierarkisk struktur i forbindelse med ekspansive kapillære senger, gir optimal perfusjon og næringstransport samtidig 11. I utgangspunktet blodkar former som en primitiv plexus som reorganiserer til en hierarkisk forgrenet nettverk under tidlig utvikling 12,13. Selv om mange av de som er involvert i disse prosessene signalene blir godt forstått 14-16, gjenstår det unnvikende hvordan en slik vaskulær mønstring bestemmes 15. I sin tur, rekapitulere denne prosessen in vitro til ingeniør organiserte vaskulære nettverk har been vanskelig. Mange eksisterende in vitro-plattformer for å modellere blodkar, for eksempel todimensjonale endoteliale cellekulturer, mangler viktige egenskaper som multi-cellulær nærhet, tre-dimensjonal geometri luminal, strømning, og ekstracellulær matriks. Tube formasjons analyser i 3D hydrogeler (kollagen eller fibrin) 17 - 19 eller invasjon analyser 20,21 har blitt brukt til å studere endotelfunksjon i 3D og deres samhandling med andre vaskulære 17,22 eller vev celletyper 23. Men samlet lumen i disse analysene mangler tilkoblinger, hemodynamisk flyt, og passende perfusjon. Videre er det tilbøyelighet for vaskulær regresjon i disse rør formasjons assays 24 hindrer at langtidskultur og modning som begrenser graden av funksjonelle studier som kan utføres. Dermed er det en gryende behov for å konstruere in vitro plattformer av mikrovaskulære nettverk som kan hensiktsmessig modellere nodothelial egenskaper og er i stand til langvarig kultur. En rekke vaskulære engineering teknikker har dukket opp gjennom årene for medisinsk bruk for å erstatte eller bypass påvirket fartøy hos pasienter med vaskulær sykdom. Stor diameter fartøy laget av syntetiske materialer slik som polyetylentereftalat (PET), og polytetrafluoretylen (ePTFE) har hatt betydelig terapeutisk suksess med langvarig åpenhet (gjennomsnittlig 95% åpenhets over 5 år) 25. Selv små diameter syntetiske transplantater (&lt;6 mm) typisk møte komplikasjoner som intimal hyperplasi og trombopoiese 26-28, vev konstruert liten diameter grafts laget med biologisk materiale har gjort betydelige fremskritt 29,30. Til tross for fremskritt av denne typen, har utviklet fartøy på mikro forble en utfordring. Å tilstrekkelig modellere mikrovaskulaturen, er det nødvendig å generere komplekse nettverksmønster med sufkelig mekanisk styrke til å opprettholde åpenhet og med en matriseblanding som gjør det mulig for både næringsgjennomtrengning for parenchymale celler og cellulære remodeling. Denne protokollen presenterer en ny kunstig perfusable fartøy nettverk som etterligner en innfødt in vivo setting med en fleksibel og kontrollerbar mikromiljøet 31-34. Den beskrevne metoden genererer konstruerte microvessels med diameter i størrelsesorden 100 mikrometer. Konstruert microvessels er fabrikkert av perfusert endotelceller gjennom et mikrofluidkanal som er innleiret i myk type I kollagen hydrogel. Dette systemet har kapasitet til å generere mønstrede nettverk med åpen luminal struktur, replikere flercellede interaksjoner, modulere ekstracellulære matrise sammensetning, og anvende fysiologisk relevante hemodynamiske krefter.

<table border="0" cellpadding="0" cellspacing="0" width="1363">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:459px;">Wafer Fabrication</td>
			<td style="width:225px;"></td>
			<td style="width:140px;"></td>
			<td style="width:540px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">AutoGlow Plasma System</td>
			<td>AutoGlow</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Headway Spin Coater</td>
			<td>Headway Research, Inc&#160;</td>
			<td colspan="2">PWM32 Spin Coater&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">ABM Contact Aligner</td>
			<td>AB-M</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Alpha Step Profilometer</td>
			<td>Tencor</td>
			<td>Alpha Step 200</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">SU-8 Developer</td>
			<td>Microchem</td>
			<td>Y020100</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">SU-8 Resist</td>
			<td>Microchem</td>
			<td>SU-8 2000</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">8&#34; silicon wafer</td>
			<td>Wafer World Inc.</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tabletop Micro Pattern Generator</td>
			<td>Heidelberg Instruments</td>
			<td>&#956;PG 101</td>
			<td>For generation of photomask</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hot plate</td>
			<td>VWR</td>
			<td>97042-646</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ispropyl alcohol</td>
			<td>Avantor Performance Materials</td>
			<td>9088</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Petri dishes (120 x 120 mm, square)</td>
			<td>Sigma-Aldrich</td>
			<td>Z617679</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trichloro(3,3,3-trifluoropropyl)silane</td>
			<td>Sigma-Aldrich</td>
			<td>MKBG3805V</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Polydimethylsiloxane (PDMS) elastomer base and curing agent</td>
			<td>Dow Corning</td>
			<td>Sylgard 184</td>
			<td>Mixed at 10:1 (w/w)</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Vacuum desiccator</td>
			<td>Sigma-Aldrich</td>
			<td>Z119024-1EA</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Oven</td>
			<td>VWR</td>
			<td>9120976</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Device Fabrication and Culture</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">poly(methyl methacrylate) (PMMA)</td>
			<td>Plexiglas</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Corona Treater</td>
			<td>Electro-Technic Products, Inc.</td>
			<td>BD-20</td>
			<td>Handheld device for plasma treatment of PMMA devices and PDMS molds</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Soldering Iron</td>
			<td>Weller&#160;</td>
			<td>WTCPS</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stainless Steel Truss Head Slotted Machine Screw</td>
			<td>McMaster-Carr&#160;</td>
			<td>91785A096</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Stainless steel dowel pins</td>
			<td>McMaster-Carr&#160;</td>
			<td>93600A060</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tweezers&#160;</td>
			<td>Miltex</td>
			<td>24-572</td>
			<td>Any similar tweezers may be used</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Spatula (Micro Spoon)</td>
			<td>Electron Microscopy Services</td>
			<td>62410-01</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Screw driver</td>
			<td></td>
			<td></td>
			<td>Any flat head screwdriver may be used, autoclaved</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Glass coverslips (22 x 22 mm)</td>
			<td>Fisher Scientific</td>
			<td>12-542B</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bleach</td>
			<td>Clorox</td>
			<td>4460030966</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Petri dishes (150 X 25mm)</td>
			<td>Corning</td>
			<td>430599</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Petri dishes (100 X 20 mm)</td>
			<td>Corning</td>
			<td>2909</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cotton, cut into 1 cm x 3 cm pieces</td>
			<td></td>
			<td></td>
			<td>Autoclaved</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Polyethyleneimine (PEI)</td>
			<td>Sigma-Aldrich</td>
			<td>P3143</td>
			<td>Dilute to 1% in cell culture grade water</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Glutaraldehyde</td>
			<td>Sigma-Aldrich</td>
			<td>G6257</td>
			<td>Dilute to 0.1% in cell culture grade water</td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;">Sterile H2O</td>
			<td></td>
			<td></td>
			<td>Autoclaved DI H2O</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Type I collagen, dissolved in 0.1% acetic acid</td>
			<td></td>
			<td></td>
			<td>Isolated from rat tails as described in Rajan et. al. 2006 (ref #37)</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">1 mL syringe</td>
			<td>BD</td>
			<td>309659</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">10 mL syringe</td>
			<td>BD</td>
			<td>309604</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">15 mL conical tubes</td>
			<td>Corning</td>
			<td>352097</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">30 mL conical tubes</td>
			<td>Corning</td>
			<td>352098</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">M199 10X Media&#160;</td>
			<td>Life Technologies</td>
			<td>11825-015</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">1N NaOH (sterile)</td>
			<td>Sigma-Aldrich</td>
			<td>415413</td>
			<td>Dilute to 1N in cell culture grade water</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">HUVECs&#160;</td>
			<td>Lonza</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Endothelial growth media</td>
			<td>Lonza</td>
			<td>CC-3124</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trypsin</td>
			<td>Corning</td>
			<td>25-052-CI</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal bovine serum (FBS)</td>
			<td>Thermofisher Scientific</td>
			<td>10082147</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dextran from Leuconostoc spp. (70kDa)</td>
			<td>Sigma-Aldrich</td>
			<td>31390</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Phosphate Buffered Saline (PBS)</td>
			<td>Corning</td>
			<td>21-031-CV</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hemocytometer</td>
			<td>Hausser Scientific Co.</td>
			<td>3200</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Gel loading tips</td>
			<td>VWR</td>
			<td>37001-152</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">18G Blunt Fill Needle</td>
			<td>BD&#160;</td>
			<td>305180</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">20G Stainless Steel Dispensing Needle</td>
			<td>McMaster-Carr</td>
			<td>75165A123</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tygon 1/32&#8221; ID, 3/32&#34; OD Silicon Tubing</td>
			<td>Cole-Parmer</td>
			<td>EW-95702-00</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">1/16&#34; Tube-to-tube Coupling</td>
			<td>McMaster-Carr</td>
			<td>5116K165</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">90&#176; Elbow Connectors, Tube-to-Tube</td>
			<td>McMaster-Carr</td>
			<td>5121K901</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Luer Lock Coupling (Female, 1/16&#34; ID)</td>
			<td>McMaster-Carr</td>
			<td>51525K211</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Plastic Forceps, with Jaw Grips</td>
			<td>Electron Microscopy Services</td>
			<td>72971</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Dual Syringe Pump</td>
			<td>Harvard Apparatus</td>
			<td>70-4505</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">5 mL Polystyrene Round-bottom tube</td>
			<td>Fisher Scientific</td>
			<td>14-959-2A</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Device Analysis</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Formaldehyde</td>
			<td>Sigma-Aldrich</td>
			<td>F8775</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Bovine Serum Albumin</td>
			<td>Sigma-Aldrich</td>
			<td>A8806-5G</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Triton X-100</td>
			<td>Sigma-Aldrich</td>
			<td>T-9284</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Rabbit anti-hCD31</td>
			<td>Abcam</td>
			<td>ab32457</td>
			<td>1:25 working dilution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">FITC conjugated anti-von Willebrand Factor antibody</td>
			<td>Abcam</td>
			<td>ab8822</td>
			<td>1:100 working dilution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Goat anti-rabbit 568 secondary antibody</td>
			<td>Thermofisher Scientific</td>
			<td>A-11011</td>
			<td>1:100 working dilution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hoescht</td>
			<td>Thermofisher Scientific</td>
			<td>H1399</td>
			<td>Resuspended in DMSO</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium cacodylate&#160;</td>
			<td>Sigma-Aldrich</td>
			<td>C0250</td>
			<td>To make 0.2M cacodylate buffer</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Ethanol</td>
			<td>VWR International</td>
			<td>BDH1164-4LP</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">40kDa FITC-conjugated Dextran</td>
			<td>Sigma-Aldrich</td>
			<td>FD40S&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Additional Culture Reagents&#160;</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">CHIR-99021</td>
			<td>Selleck Chem</td>
			<td>S2924</td>
			<td>Small molecule GSK-3 inhibitor</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Human recombinant VEGF</td>
			<td>Peprotech</td>
			<td>100-20</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Human recombinant bFGF</td>
			<td>Peprotech</td>
			<td>AF-100-18B</td>
			<td></td>
		</tr>
	</tbody>
</table>

micropatterning and assembly of 3d microvessels

In vitro-plattformer for å studere endotelceller og vaskulær biologi er i stor grad begrenset til 2D endotelial cellekultur, strømningskamre med polymer- eller glassbaserte substrater, og hydrogel-baserte rør formasjons analyser. Disse analysene, mens informativ, ikke rekapitulere lumen geometri, riktig ekstracellulære matrise, og multi-cellular nærhet, som spiller sentrale roller i moduler vaskulær funksjon. Dette manuskriptet beskriver en sprøytestøpemetode for å generere konstruerte fartøyer med diameter i størrelsesorden 100 mikrometer. Microvessels er fabrikkert ved såing av endotelceller i et mikrofluidkanal integrert i en naturlig type I kollagen hydrogel. Ved å innlemme parenkymceller i kollagenmatriksen før kanaldannelse kan spesifikke vev microenvironments modelleres og undersøkt. Ytterligere modulasjoner av hydrodynamiske egenskaper og media sammensetning gir mulighet for styring av komplekse vaskulær funksjon innenfor den ønskede mikromiljøet.Denne plattformen gjør det mulig for studiet av perivaskulær celle rekruttering, blod-endotel interaksjoner, strømrespons, og vev-mikrovaskulær interaksjoner. Konstruert microvessels tilbyr muligheten til å isolere påvirkningen fra enkelte komponentene i en vaskulær nisje og presist kontrollere dens kjemiske, mekaniske og biologiske egenskaper til å studere vaskulær biologi i både helse og sykdom.

Den konstruerte fartøy plattformen skaper funksjonelle microvasculature integrert i en naturlig kollagen type I matrise og gir stram kontroll over mobilnettet, biofysiske og biokjemiske miljø in vitro. Å dikte konstruert microvessels er menneskenavlevene endotelceller (HUVECs) perfusert gjennom kollagen-embedded microfluidic nettverk hvor de skal festes for å danne et patent lumen og sammenflytende endotelet. Som illustrert i figur 1A-C, kan fartøyet geomet...

Watch this Scientific Journal Video about Micropatterning and Assembly of 3D Microvessels at JoVE.com

Micropatterning and Assembly of 3D Microvessels

Dette manuskriptet presenterer en injeksjon metode for å konstruere microvessels at rekapitulere fysiologiske egenskapene til endotelet. Den mikrofluidbasert prosess skaper patent 3D-vaskulære nettverk med tailorable tilstander, slik som flyt, cellesammensetning, geometri, og biokjemiske gradienter. Fremstillingsprosessen og eksempler på potensielle anvendelser beskrives.

Micropatterning og montering av 3D microvessels

In vitro platforms to study endothelial cells and vascular biology are largely limited to 2D endothelial cell culture, flow chambers with polymer or glass ...

Research

JoVE Journal

Bioengineering

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Utarbeidelse av 3D kollagen Gels og mikro for studier av 3D Interaksjoner<em&gt; I Vivo</em

Historically, most cellular processes have been studied in only 2 dimensions.  While these studies have been informative about general cell signaling ...

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automatisert robot flytende håndtering samlingen av modulære DNA enheter

Recent advances in modular DNA assembly techniques have enabled synthetic biologists to test significantly more of the available &#34;design space&#34; ...

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Syntese av kationiserte Magnetoferritin for Ultra-rask Magnetization Celler

Many important biomedical applications, such as cell imaging and remote manipulation, can be achieved by labeling cells with superparamagnetic iron oxide ...

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Bioprinting brusk og huden vev Analogs utnytte en roman passiv miksing enhet teknikk for Bioink Precellularization

Bioprinting is a powerful technique for the rapid and reproducible fabrication of constructs for tissue engineering applications. In this study, both ...

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Fabrikasjon av egendefinerte Agarose for celle såing og vev Ring selvstendig montering med 3D-trykt muggsopp

Engineered tissues are being used clinically for tissue repair and replacement, and are being developed as tools for drug screening and human disease ...

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

Protokoller av 3D Bioprinting av gelatin Methacryloyl hydrogel basert Bioinks

Gelatin methacryloyl (GelMA) has become a popular biomaterial in the field of bioprinting. The derivation of this material is gelatin, which is hydrolyzed ...

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

Temperaturkontrollert montering og karakterisering av et dråpegrensesnitt Bilayer

The droplet interface bilayer (DIB) method for assembling lipid bilayers (i.e., DIBs) between lipid-coated aqueous droplets in oil offers key benefits ...

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Kontroll av cellegeometri gjennom infrarød laserassistert mikropatterning

Micropatterning is an established technique in the cell biology community used to study connections between the morphology and function of cellular ...

A Micropatterning Assay for Measuring Cell Chirality

En mikropatterningsanalyse for måling av cellekoralitet

Chirality is an intrinsic cellular property, which depicts the asymmetry in terms of polarization along the left-right axis of the cell. As this unique ...

Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting

Gelatinmetakryloyl granulære hydrogelstillas: Mikrogelfabrikasjon med høy gjennomstrømning, lyofilisering, kjemisk montering og 3D-bioprinting

The emergence of granular hydrogel scaffolds (GHS), fabricated via assembling hydrogel microparticles (HMPs), has enabled microporous scaffold formation ...