Name: ferric chloride-induced murine thrombosis models
Uploaded: 2016-09-05T13:00:00
Description: Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

This work was supported by the National Heart Lung and Blood Institute (NHLBI) of the National Institutes of Health under award numbers R01 HL121212 (PI: Sen Gupta), R01 HL129179 (PI: Sen Gupta, Co-I: Li) and R01 HL098217 (PI: Nieman). The content of this publication is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

وقد تمت الموافقة على جميع الإجراءات والتلاعب من الحيوانات من قبل المؤسسات ورعاية الحيوان واللجان الاستخدام (IACUC) من كليفلاند كلينيك وفقا لسياسة الولايات المتحدة الأمريكية خدمة الصحة العامة لرعاية الرفق بالحيوان واستخدام الحيوانات، ودليل المعاهد الوطنية للصحة <...

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3 -induced نموذج FeCl هو واحد من نماذج تجلط الدم الأكثر استخداما، والتي يمكن أن توفر معلومات قيمة حول التعديلات الجينية على وظيفة الصفائح الدموية وتخثر 7،8،16،19،31-33 فحسب، بل يمكن أيضا أن تكون أداة قيمة لتقييم المركبات والاستراتيجيات العلاجية للعلاج والوقاية من ?...

تجلط الدم الشرياني (تجلط الدم) هو اختلاط شائع في العديد من الأمراض الجهازية المرتبطة التهاب مزمن، بما في ذلك تصلب الشرايين والسكري والسمنة والسرطان واضطرابات المناعة الذاتية الروماتيزم المزمنة. وتورط الجلطات التي تحدث في الجذعية الدموية في الشرايين من نشاط الصفائح الدموية غير مناسب، وتجميع وآليات coagulatory اللاحقة، والنوبات القلبية والسكتات الدماغية وفقدان أقصى. جدار الوعاء الدموي هو نظام معقد يتضمن أنواع خلايا متعددة ويتأثر عدد كبير من العوامل الخارجية بما في ذلك إجهاد القص، خلايا الدم، والهرمونات، والسيتوكينات، وكذلك التعبير عن البروتينات المضادة للأكسدة في جدار الوعاء الدموي. وفي التجارب المختبرية لا يمكن نسخ هذه البيئة المعقدة، وبالتالي الدراسات المجراة باستخدام نماذج حيوانية حاسمة للسماح فهم أفضل الآليات التي تشارك في اضطرابات الجلطات. وقد أظهرت الفئران أن يكون سيمآليات ILAR للبشر من حيث تجلط الدم وتصلب الشرايين، والتهاب 1،2 السكري. وعلاوة على ذلك، المعدلة وراثيا وخروج المغلوب الفئران يمكن أن تنشأ لاختبار وظيفة من المنتجات جين معين في فيزيولوجي معقدة أو بيئة مرضية. مثل هذه الدراسات تحاكي أمراض البشر ويمكن أن توفر المعلومات الآلية هامة تتعلق اكتشاف مسارات والعلاجات، جديدة، فضلا عن توفر تفاصيل مهمة في وصف آثار المخدرات على تجلط الدم. تحدث بسبب البطانية إصابة طبقة أو اختلال وظيفي والتعرض للتيار الدم إلى مصفوفة تحت البطانة 3،4 الجلطة الدموية في الشرايين المرضية. وقد وضعت نماذج مختلفة تخثر للحث على هذا الضرر البطانية مثل الإصابة الميكانيكية، وإصابة الأكسدة مقرها ولاية البنغال-مجمع photoreactive روز وإصابة الليزر 5. في هذا الطيف، كلوريد الحديديك (FeCl 3) -induced إصابة الأوعية الدموية هو النموذج المستخدم على نطاق واسع من تجلط الدم. هذا كاشف عندمايطبق على الجانب الخارجي من السفن يدفع الضرر التأكسدي للخلايا الأوعية الدموية 6-8، مع فقدان الحماية الخلايا البطانية من تعميم الصفائح الدموية ومكونات شلال التخثر. نموذج FeCl 3 بسيط وحساس لكلا تخثر ومعاداة الصفائح الدموية المخدرات، وقد تم تنفيذها على الشريان السباتي والشرايين الفخذية، وعروق الوريد، والمساريقي والشرايين والأوردة العضلة المشمرة في الفئران والجرذان والخنازير الغينية والأرانب 6-15. واحد المعلمة قابلة للقياس في هذا النموذج هو الوقت المنقضي من الاصابة لاستكمال انسداد الأوعية يقاس وقف تدفق الدم مع تدفق متر دوبلر أو تحت الملاحظة المباشرة مع 6،7،9 intravital المجهري. تم الإبلاغ عن مجموعة من الأوقات ما بين 5 إلى 30 دقيقة في دراسات مختلفة في C57Bl6 الفئران 7-10،16، مما يوحي بأن FeCl 3 تركيزات، وأنواع التخدير، والتقنيات الجراحية والعمر الماوس، خلفية الجينومية، وطريقة قياس بتدفق lood، والمتغيرات البيئية الأخرى يكون لها آثار كبيرة في هذا النموذج. هذا التباين واسع يجعل من الصعب المقارنة بين الدراسات من المجموعات البحثية المختلفة وقد جعل الكشف عن الفروق الدقيقة صعوبة. مع رؤية للحد من هذه المتغيرات، وإقامة قابلة للتكرار بشكل موحد في نظام نموذج الجسم الحي، ولقد صقل نموذج الشريان السباتي -induced FeCl 3 الذي يجعل بيانات استنساخه للغاية مع الحد الأدنى من الاختلاف 6-10،16-19. في هذه الورقة وصفنا وتبادل المهارات وتقديم تقارير عدة أمثلة تجريبية التمثيلية التي يمكن أن تستفيد من هذا النموذج.

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			<td>to dissect and separate soft tissue</td>
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			<td>to dissect and separate soft tissue</td>
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			<td>P3510</td>
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			<td height="40" style="height:40px;width:351px;">Medline Surgical Instrument Sterilization Steam Autoclave Tapes</td>
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			<td>111625</td>
			<td>To fix the mouse to the plate</td>
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			<td>For sterlization of solutions injected to mice</td>
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			<td height="26" style="height:26px;width:351px;">Fisherbrand&#8482;&#160;Syringe Filters - Sterile 0.45&#181;m</td>
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			<td>To filter the FeCl3 solution</td>
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			<td height="20" style="height:20px;width:351px;">Agarose&#160;</td>
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ferric chloride-induced murine thrombosis models

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, diabetes, obesity, cancer and chronic autoimmune rheumatologic disorders. Thrombi are the cause of most heart attacks, strokes and extremity loss, making thrombosis an extremely important public health problem. Since these thrombi stem from inappropriate platelet activation and subsequent coagulation, targeting these systems therapeutically has important clinical significance for developing safer treatments. Due to the complexities of the hemostatic system, in vitro experiments cannot replicate the blood-to-vessel wall interactions; therefore, in vivo studies are critical to understand pathological mechanisms of thrombus formation. To this end, various thrombosis models have been developed in mice. Among them, ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation and aggregation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for the development of a thrombus that occludes blood flow gives a quantitative measure of vascular injury, platelet activation and aggregation that is relevant to thrombotic diseases. We have significantly refined this FeCl3-induced vascular thrombosis model, which makes the data highly reproducible with minimal variation. Here we describe the model and present representative data from several experimental set-ups that demonstrate the utility of this model in thrombosis research.

الشريان السباتي التخثر نموذج في الفئران مع C57BL6 الخلفية، نوصي باستخدام 7.5٪ FeCl 3 لعلاج السفينة لمدة 1 دقيقة كنقطة انطلاق. تحت العلاج من 7.5٪ FeCl 3، يتم تحديد حدود المنطقة الجرحى وجدار الوعاء الدموي العادي بسهولة تحت المجهر (انظر <st...

Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

Ferric Chloride-induced Murine Thrombosis Models

نفيدكم إجراء المكرر من كلوريد الحديديك (FeCl 3) نماذج تخثر -induced على الشريان السباتي والمساريقي الشريان وكذلك السياق، تتميز بكفاءة استخدام intravital المجهري لمراقبة الوقت لالكاتمة للتكوين الجلطة الدموية.

الحديديك كلوريد الناجم عن الفئران التخثر نماذج

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, ...

Research

JoVE Journal

Medicine

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis

Electrolytic Inferior Vena Cava Model EIM of Venous Thrombosis

كهربائيا الوريد الأجوف السفلي النموذجي (EIM) من التخثر الوريدي

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential ...

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

الفأرة النموذجي ركود كاملة من الوريد الأجوف السفلي التخثر

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

تجزئة والقياس من وحدات تخزين الدهون في الفئران نماذج السمنة عن طريق الأشعة السينية التصوير المقطعي

Obesity is associated with increased morbidity and mortality as well as reduced metrics in quality of life.1 Both environmental and genetic factors are ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

حقن Intrastriatal من ذاتي الدم أو اللاهوائية كولاجيناز كنماذج الفئران من نزف الدماغي الداخلي

Intracerebral hemorrhage (ICH) is a common form of cerebrovascular disease and is associated with significant morbidity and mortality. Lack of effective ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

حقن مثلي من خلايا سرطان الثدي في الثديية الدهون وسادة من الفئران لدراسة نمو الأورام.

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

التكيف Videofluoroscopic طرق دراسة السنونو الإنسان لكشف وتوصيف عسر البلع في نماذج الفئران الأمراض

This study adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating ...

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

عالية التردد التصوير بالموجات فوق الصوتية للماوس عنق الرحم الغدد الليمفاوية

High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal ...

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

بروتوكول MRI متعددة المراكز لتقييم النوعي والكمي للجلطة

We evaluated a magnetic resonance venography (MRV) approach with gadofosveset to quantify total thrombus volume changes as the principal criterion for ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

الحديديك تخثر نموذج الفأر على الشريان السباتي ومساريق السفينة التي يسببها كلوريد

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...