Name: ferric chloride-induced murine thrombosis models
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Description: Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

This work was supported by the National Heart Lung and Blood Institute (NHLBI) of the National Institutes of Health under award numbers R01 HL121212 (PI: Sen Gupta), R01 HL129179 (PI: Sen Gupta, Co-I: Li) and R01 HL098217 (PI: Nieman). The content of this publication is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

모든 절차와 동물의 조작은 미국 공중 보건 서비스 동물 애호 관리에 대한 정책 및 동물의 사용 및 관리에 대한 NIH 가이드 및에 따라 클리블랜드 클리닉의 기관 동물 관리 및 사용위원회 (IACUC)에 의해 승인되었습니다 실험 동물의 사용. 1. 준비 : <ol><li> 라벨 혈소판에 대한 형광 염료 <ol><li> 식염수에, 로다 민 6G 솔루션, 0.5 ㎎ / ㎖를 준비하고 0.22 μm의...

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50ml을 유도 된 모델은 혈소판 기능 및 혈전증 7,8,16,19,31-33의 유전자 변형에 대한 유용한 정보를 제공 할뿐만 아니라, 가장 널리 사용되는 혈전 모델의 하나이며, 또한 유용한 도구가 될 수있다 죽상 혈전증 질환 11,17,34-37의 치료 및 예방을위한 치료 화합물과 전략의 평가. 여기에서 우리는 우리의 수정이 모델의 개선을 표시하고 항응고제 (TPA) 및 항 혈소판 약물 (PAR4 항체)?...

동맥 혈전증 (혈전)는 죽상 동맥 경화증, 당뇨병, 비만, 암,자가 면역 만성 류마티스 질환을 포함한 만성 염증과 관련된 다양한 전신 질환의 일반적인 합병증이다. 부적절한 혈소판 활성화, 집계 이후 coagulatory 메커니즘에서 동맥 순환 줄기에 발생하며 혈전은 심장 마비, 뇌졸중 및 사지 ​​손실에 연루되어있다. 혈관벽은 혈관벽에 혈액 세포, 호르몬 및 사이토킨뿐만 아니라 항산화 단백질의 발현을 순환하는 다수의 세포 유형을 포함하고, 전단 응력을 포함 외적 요인의 다수에 의해 영향 복잡한 시스템이다. 시험 관내 실험에서 복제 할 수없는 이 복잡한 환경 때문에 동물 모델을 이용한 생체 내 연구는 혈전 성 질환에 관여하는 메커니즘을 더 잘 이해할 수 있도록하는 것이 중요하다. 마우스는 SIM이 밝혀졌다혈전증, 동맥 경화, 염증과 당뇨병 1,2의 관점에서 인간 ILAR 메커니즘. 또한, 유전자 녹아웃 마우스는 복잡한 생리 학적 또는 병리학 적 환경에서 특정 유전자 산물의 기능을 테스트하기 위해 생성 될 수있다. 이러한 연구는 인간의 병리를 모방하고 새로운 경로 및 치료법의 발견에 관한 중요한 기계론의 정보를 제공 할뿐만 아니라 혈전증에 약물 효과를 특징 짓는 중요한 정보를 제공 할 수있다. 병적 동맥 혈전은 피하 매트릭스 3,4에 층을 손상 또는 기능 장애와 혈류의 노출을 내피로 인해 발생합니다. 다양한 혈전증 모델 기계적 손상, 광 반응성 화합물 로즈 벵갈 계 산화 적 손상 및 레이저 손상이 5로 내피 세포의 손상을 유도하기 위해 개발되었다. 이 스펙트럼에서는, 철 클로라이드 (50ml을 3) 혈관 손상은 혈전증 널리 사용 유도 된 모델이다. 이 시약 때용기의 외 측면에인가 된 혈소판 및 응고 캐스케이드의 요소를 순환에서 내피 세포의 보호 상실, 혈관 세포 6-8에 산화 손상을 유발한다. 50ml을 모델은 간단하고 모두 항응고제 및 항 혈소판 약물에 민감하고, 쥐, 쥐, 기니피그와 토끼 6-15에서 경동맥과 대퇴 동맥, 경정맥 및 장간막과 cremasteric 세동맥과 세정맥에 수행되었습니다. 이 모델에서 하나의 측정 매개 변수는 도플러 유량계 또는 생체 내에 현미경 6,7,9 직접 관찰에서 혈류의 중단으로 측정 혈관 폐색을 완료 부상에서 경과 된 시간입니다. 5 ~ 30 분 사이의 시간의 범위를 제안 C57BL6 마우스 7-10,16 다른 연구에서보고 된 50ml을 그 농도 마취 수술 기법 마우스 연령, 게놈 배경 B의 측정 방법의 종류흐름들 (100d), 및 기타 환경 변수는이 모델에서 중요한 효과를 가지고있다. 이 넓은 다양성 어려운 다른 연구 그룹에서 연구를 비교하고 미묘한 차이의 검출이 어려워 질 수 있습니다. 이러한 변화 성을 최소화하고 생체 내 모델 시스템에서 균일하게 재현을 확립하는 시각과 함께, 우리는 최소의 변형 6-10,16-19 매우 재현성있는 데이터를 만든다 50ml을 유도 된 경동맥 모델을 정제했다. 본 논문에서는 설명하고 기술을 공유하고이 모델에서 혜택을 누릴 수있는 몇 가지 대표적인 실험 예를보고한다.

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			<td>11018-12</td>
			<td>cut and hold skin</td>
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			<td height="40" style="height:40px;width:351px;">Metzenbaum Fino Scissors - Tungsten Carbide/Curved/Blunt-Blunt/14.5cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td style="width:119px;">14519-14&#160;&#160;</td>
			<td>to dissect and separate soft tissue</td>
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			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>13021-12</td>
			<td>to dissect and separate soft tissue</td>
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			<td height="20" style="height:20px;">Graefe Forceps - Serrated/Straight/10cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td style="width:119px;">11050-10</td>
			<td>to dissect and separate soft tissue</td>
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			<td>11254-20&#160;</td>
			<td>Isolate vessel from surounding tissue</td>
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			<td>Isolate vessel from surounding tissue</td>
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			<td>Needle holders</td>
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			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>18020-40</td>
			<td>For fix the incisors to the plate</td>
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			<td height="20" style="height:20px;width:351px;">Suture Thread 6-0</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
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			<td>For all surgery and ligation</td>
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			<td height="20" style="height:20px;">rhodamine 6G&#160;</td>
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			<td>To lebel platelets</td>
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			<td style="width:119px;">12321</td>
			<td>To induce vessel injury</td>
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			<td height="20" style="height:20px;">Papaverine hydrochloride</td>
			<td style="width:159px;">Sigma</td>
			<td>P3510</td>
			<td>To inhibit gut peristalsis.</td>
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			<td height="40" style="height:40px;width:351px;">Medline Surgical Instrument Sterilization Steam Autoclave Tapes</td>
			<td style="width:159px;">Medline</td>
			<td>111625</td>
			<td>To fix the mouse to the plate</td>
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			<td height="20" style="height:20px;width:351px;">Fisherbrand&#8482;&#160;Syringe Filters - Sterile 0.22&#181;m</td>
			<td style="width:159px;">Fisher</td>
			<td>09-720-004</td>
			<td>For sterlization of solutions injected to mice</td>
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			<td height="26" style="height:26px;width:351px;">Fisherbrand&#8482;&#160;Syringe Filters - Sterile 0.45&#181;m</td>
			<td style="width:159px;">Fisher</td>
			<td>09-719D</td>
			<td>To filter the FeCl3 solution</td>
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			<td height="20" style="height:20px;width:351px;">Sterile Alcohol Prep Pad</td>
			<td style="width:159px;">Fisher</td>
			<td>06-669-62</td>
			<td>To sterilize the surgical site</td>
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			<td height="20" style="height:20px;width:351px;">Agarose&#160;</td>
			<td style="width:159px;">BioExpress</td>
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			<td>To make gel stage</td>
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			<td height="20" style="height:20px;width:351px;">Leica DMLFS fluorescent microscope</td>
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			<td>Intravital microscope</td>
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			<td height="40" style="height:40px;width:351px;">GIBRALTAR Platform and X-Y Stage System</td>
			<td style="width:159px;">npi electronic GmbH</td>
			<td style="width:119px;"></td>
			<td style="width:325px;">http://www.npielectronic.de/products/micropositioners/burleigh/gibraltar.html</td>
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			<td height="20" style="height:20px;width:351px;">Streampix version 3.17.2 software</td>
			<td style="width:159px;">NorPix</td>
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ferric chloride-induced murine thrombosis models

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, diabetes, obesity, cancer and chronic autoimmune rheumatologic disorders. Thrombi are the cause of most heart attacks, strokes and extremity loss, making thrombosis an extremely important public health problem. Since these thrombi stem from inappropriate platelet activation and subsequent coagulation, targeting these systems therapeutically has important clinical significance for developing safer treatments. Due to the complexities of the hemostatic system, in vitro experiments cannot replicate the blood-to-vessel wall interactions; therefore, in vivo studies are critical to understand pathological mechanisms of thrombus formation. To this end, various thrombosis models have been developed in mice. Among them, ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation and aggregation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for the development of a thrombus that occludes blood flow gives a quantitative measure of vascular injury, platelet activation and aggregation that is relevant to thrombotic diseases. We have significantly refined this FeCl3-induced vascular thrombosis model, which makes the data highly reproducible with minimal variation. Here we describe the model and present representative data from several experimental set-ups that demonstrate the utility of this model in thrombosis research.

경동맥의 동맥 혈전증 모델 C57BL6 배경으로 마우스에서 우리는 출발점으로 1 분 동안 혈관을 치료하기 위해 7.5 % 50ml을 사용하는 것이 좋습니다. 7.5 %의 치료 50ml을에서 부상 영역과 정상 혈관 벽의 경계는 쉽게 (참조 온라인 비디오 1) 현미경으로 식별, 내피 층이 크게 손상되었음을 시사한다. 혈전은 50ml을 처리 즉시 ?...

Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

Ferric Chloride-induced Murine Thrombosis Models

우리는 혈전 형성을 폐쇄하는 시간을 모니터링하는 생체 내에 현미경을 사용하여 효율적 특징으로하는 정제 된 철 클로라이드 (50ml을) 경동맥 및 장간막 동맥에 유도 된 혈전증 모델의 절차뿐만 아니라 정맥을보고한다.

철 염화물에 의한 쥐 혈전증 모델

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, ...

Research

JoVE Journal

Medicine

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis

Electrolytic Inferior Vena Cava Model EIM of Venous Thrombosis

정맥 혈전증의 전해 하부 대정 맥 모델 (EIM)

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential ...

연구

의학

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

하부 대정 맥 혈전증의 마우스 완전한 스테이 시스 모델

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

세분화 및 Murine 비만 모델에서 지방 볼륨 측정 X 선 계산된 Tomography를 사용하여

Obesity is associated with increased morbidity and mortality as well as reduced metrics in quality of life.1 Both environmental and genetic factors are ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

쥐의 대동맥 부비동의 동맥 경화 병변의 정량 분석​​ 및 특성

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

뇌출혈의 쥐과 모델로자가 혈액이나 클로스 트리 디움 콜라게나 제의 줄무늬 체 주입

Intracerebral hemorrhage (ICH) is a common form of cerebrovascular disease and is associated with significant morbidity and mortality. Lack of effective ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

마우스의 유방 지방 패드에 유방암 세포의 소성 주입은 종양의 성장을 연구한다.

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

인간 Videofluoroscopic 삼키기 연구 방법을 적응하는 것은 검색 및 설치류 질병 모델에서 연하의 특성을

This study adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating ...

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

마우스 경부 림프절의 고주파 초음파 이미징

High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal ...

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

깊은 정맥 혈전증의 평가 및 정량화를위한​​ 다기관 MRI 프로토콜

We evaluated a magnetic resonance venography (MRV) approach with gadofosveset to quantify total thrombus volume changes as the principal criterion for ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

철 염화물에 의한 경동맥 및 장간막 용기에 혈전증 마우스 모델

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...