Name: ferric chloride-induced murine thrombosis models
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Description: Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

This work was supported by the National Heart Lung and Blood Institute (NHLBI) of the National Institutes of Health under award numbers R01 HL121212 (PI: Sen Gupta), R01 HL129179 (PI: Sen Gupta, Co-I: Li) and R01 HL098217 (PI: Nieman). The content of this publication is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Все процедуры и манипуляции животных были одобрены Институциональные уходу и использованию животных комитетов (IACUC) из клиники Кливленда в соответствии с политикой Соединенных Штатов службы общественного здравоохранения на гуманное лечение и использовании животного мира , а также в <em...

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FeCl 3 индуцированную модель является одним из наиболее широко используемых моделей тромбоз, который может не только предоставить ценную информацию о генетических модификаций на функции тромбоцитов и тромбоза 7,8,16,19,31-33, но также может быть ценным инструментом для оценки тер?...

Артериальный тромбоз (сгусток крови) является частым осложнением многих системных заболеваний, связанных с хроническим воспалением, включая атеросклероз, сахарный диабет, ожирение, рак и хронические аутоиммунные ревматологических заболеваний. Тромбы, которые происходят в стволе артериальной циркуляции с несоответствующей активацией тромбоцитов, агрегации и последующих coagulatory механизмов, а также участвуют в сердечных приступов, инсультов и потери конечностей. Стенка сосуд представляет собой сложную систему , которая включает в себя различные типы клеток и зависит от множества внешних факторов , в том числе напряжения сдвига, циркулирующих в крови клеток, гормоны и цитокины, а также экспрессию антиоксидантных белков в стенке сосуда. В пробирке эксперименты не может реплицировать это сложная среда , и поэтому в естественных условиях исследования с использованием животных моделей имеют решающее значение для обеспечения лучшего понимания механизмов , участвующих в тромботических заболеваний. Мыши было показано, что симИЛАР механизмы для человека с точки зрения тромбоз, атеросклероз, воспаление и диабета 1,2. Кроме того, трансгенные и нокаутные мыши могут быть созданы для тестирования функции специфических генных продуктов в сложном физиологическом или патологическом среде. Такие исследования имитировать патологии человека и может предоставить важную механистическую информацию, связанную с открытием новых путей и методов лечения, а также обеспечивают важные детали при характеристике эффекты препарата на тромбоз. Патологические артериальных тромбов происходят из - за эндотелиального повреждения слоя или дисфункции и воздействия на поток крови к субэндотелиальном матрицы 3,4. Различные модели тромбоз, были разработаны , чтобы вызвать это повреждение эндотелия , таких как механическое повреждение, фотореакционноспособная соединение бенгальский розовый на основе окислительного повреждения и лазерной травмы 5. В этом спектре, хлорид железа (FeCl 3) индуцированный сосудистых повреждений является широко используемой моделью тромбоза. Этот реагент, когдаприкладывается к внешней аспекту сосудов вызывает окислительное повреждение клеток сосудов 6-8, с потерей защиты эндотелиальных клеток от циркулирующих тромбоцитов и компонентов каскада свертывания. Модель FeCl 3 проста и чувствительна к обоим антикоагулянта и анти-Тромбоциты препаратов, и было выполнено на сонных и бедренных артерий, яремной вены, и брыжеечных и cremasteric артериолы и венулы у мышей, крыс, морских свинок и кроликов 6-15. Один измеряемый параметр в этой модели является то, время , прошедшее после травмы до полной окклюзии сосуда, измеряемое как прекращение кровотока с помощью измерителя потока Доплера или под непосредственным наблюдением с прижизненной микроскопии 6,7,9. Диапазон времени от 5 до 30 мин, как сообщается в различных исследованиях в C57BL6 мышей 7-10,16, предполагая , что концентрации FeCl 3, виды анестезии, хирургических методов, мыши возраста, геномную фона, метод измерения BLOOD потока, а также других параметров окружающей среды оказывают значительное влияние в этой модели. Эта широкая изменчивость затрудняет сравнивать исследования из различных исследовательских групп и может сделать обнаружение тонких различий трудно. С видением , чтобы свести к минимуму такие изменчивости и установить равномерно воспроизводимые в модели естественных условиях системы, мы усовершенствовали модель артерии индуцированная сонной FeCl 3 , что делает данные с высокой воспроизводимостью с минимальным изменением 6-10,16-19. В этой статье мы описываем и делиться навыками и сообщают о нескольких репрезентативных экспериментальных примеров, которые могут извлечь выгоду из этой модели.

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ferric chloride-induced murine thrombosis models

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, diabetes, obesity, cancer and chronic autoimmune rheumatologic disorders. Thrombi are the cause of most heart attacks, strokes and extremity loss, making thrombosis an extremely important public health problem. Since these thrombi stem from inappropriate platelet activation and subsequent coagulation, targeting these systems therapeutically has important clinical significance for developing safer treatments. Due to the complexities of the hemostatic system, in vitro experiments cannot replicate the blood-to-vessel wall interactions; therefore, in vivo studies are critical to understand pathological mechanisms of thrombus formation. To this end, various thrombosis models have been developed in mice. Among them, ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation and aggregation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for the development of a thrombus that occludes blood flow gives a quantitative measure of vascular injury, platelet activation and aggregation that is relevant to thrombotic diseases. We have significantly refined this FeCl3-induced vascular thrombosis model, which makes the data highly reproducible with minimal variation. Here we describe the model and present representative data from several experimental set-ups that demonstrate the utility of this model in thrombosis research.

Сонная артерия Тромбоз Модель У мышей с C57BL6 фоне, мы рекомендуем использовать 7,5% FeCl 3 для лечения сосуда в течение 1 мин в качестве отправной точки. Под обработкой 7,5% FeCl 3, границы поврежденной области и нормальной стенки сосуда легко идентифици?...

Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

Ferric Chloride-induced Murine Thrombosis Models

Мы сообщаем о изысканную процедуру хлорида железа (FeCl 3) индуцированный моделей тромбоз на сонной и брыжеечной артерии, а также вены, характеризующийся эффективно использовать прижизненной микроскопии для контроля времени окклюзионные тромбообразования.

Хлорного железа-индуцированной мышиной модели Тромбоз

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, ...

Research

JoVE Journal

Medicine

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis

Electrolytic Inferior Vena Cava Model EIM of Venous Thrombosis

Электролитические нижней полой вены Model (EIM) венозного тромбоза

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential ...

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

Мышь Полная модель Стасис из Нижнего тромбоз полой вены

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

Сегментация и измерение объемов жира в мышиной модели ожирения Использование рентгеновской компьютерной томографии

Obesity is associated with increased morbidity and mortality as well as reduced metrics in quality of life.1 Both environmental and genetic factors are ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Количественный анализ и характеристика атеросклеротических поражений в синусе аорты мышиной

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

Intrastriatal Инъекция аутологичной крови или клостридиальной коллагеназы как мышиный Модели внутримозгового кровоизлияния

Intracerebral hemorrhage (ICH) is a common form of cerebrovascular disease and is associated with significant morbidity and mortality. Lack of effective ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

Ортотопическая инъекции клеток рака молочной железы в молочной жировой ткани мышей для изучения роста опухоли.

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

Адаптация человека Videofluoroscopic Ласточка изучит методы обнаруживать и определять характеристики Дисфагия в мышиных Disease Models

This study adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating ...

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

Высокочастотный ультразвуковой визуализации мыши шейных лимфатических узлов

High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal ...

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

Многоцентровое МРТ Протокол оценки и количественного определения тромбоза глубоких вен

We evaluated a magnetic resonance venography (MRV) approach with gadofosveset to quantify total thrombus volume changes as the principal criterion for ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

Хлорного железа, вызванной Тромбоз мышь Модель на сонной артерии и брыжейки судна

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...