Name: ferric chloride-induced murine thrombosis models
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Description: Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

This work was supported by the National Heart Lung and Blood Institute (NHLBI) of the National Institutes of Health under award numbers R01 HL121212 (PI: Sen Gupta), R01 HL129179 (PI: Sen Gupta, Co-I: Li) and R01 HL098217 (PI: Nieman). The content of this publication is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Tüm prosedürler ve hayvanların manipülasyonlar Amerika Birleşik Devletleri Halk Sağlığı Servisi İnsani Bakım Politika ve Hayvanların Kullanımı ve Bakımı NIH Kılavuzu ve uygun Cleveland Clinic Kurumsal Hayvan Bakım ve Kullanım Komiteleri (IACUC) tarafından onaylanmıştır Laboratuar Hayvanlarının kullanımı. 1. Hazırlıklar: <ol><li> Etiketleme plateletler için floresan boya <ol><li> tuzlu su içinde, rodamin 6G çözeltisi, 0.5 mg /...

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FeCl3 indüklediği modeli trombosit fonksiyonu ve tromboz 7,8,16,19,31-33 genetik değişiklikler hakkında değerli bilgiler sağlayabilir sadece en yaygın olarak kullanılan tromboz modellerinde biridir, ama aynı zamanda değerli bir araç olabilir aterotrombotik hastalıkların 11,17,34-37 tedavisi ve önlenmesi için terapötik bileşikler ve stratejiler değerlendirilmesi için. Burada biz modifikasyonları ve bu modelin iyileştirmeler gösterildiği antikoagülan (tPA), anti-tr...

Arter trombozu (kan pıhtısı) ateroskleroz, diyabet, obezite, kanser ve kronik otoimmün romatolojik bozukluklar gibi kronik inflamasyon ile ilişkili birçok sistemik hastalığın sık görülen bir komplikasyondur. uygunsuz trombosit aktivasyonu, agregasyonu ve sonraki coagulatory mekanizmalarından arteriyel dolaşım kök meydana ve trombüs kalp krizi, felç ve ekstremite kaybına neden oluyor. Damar duvarı damar duvarındaki kan hücreleri, hormonlar ve sitokinler yanı sıra antioksidan proteinlerin ifadesini dolaşan, birden fazla hücre tiplerini içerir ve kayma gerilmesi dahil dışsal faktörlerin çok sayıda etkilenir karmaşık bir sistemdir. In vitro deneyler çoğaltma yapamaz Bu kompleks bir ortam ve bu nedenle, hayvan modelleri kullanarak in vivo çalışmalar, trombotik hastalıklarda yer alan mekanizmaların daha iyi anlaşılması izin vermek için çok önemlidir. Fareler sim sahip olduğu gösterilmiştirtromboz, ateroskleroz, enflamasyon ve diyabet 1,2 açısından insanlara Ilar mekanizmaları. Ayrıca, transjenik ve knockout farelerde kompleks fizyolojik veya patolojik bir ortamda belirli bir gen ürünlerinin işlevi test etmek için oluşturulabilir. Bu tür çalışmalar, insan patoloji taklit ve yeni yollar ve tedavilerin, keşif ile ilgili önemli mekanistik bilgi vermek yanı sıra tromboz uyuşturucu etkileri karakterize önemli ayrıntıları sağlayabilir. Patolojik arteriyel trombüs subendotelyal matrise 3,4 katman yaralanma veya disfonksiyon ve kan akışının pozlama endotel nedeniyle oluşur. Çeşitli tromboz modelleri, mekanik yaralanma, photoreactive bileşik Rose Bengal tabanlı oksidatif hasarı ve lazer yaralanması 5 olarak bu endotel hasarına neden geliştirilmiştir. Bu spektrumda, Demir klorür (FeCl3) vasküler yaralanma tromboz yaygın olarak kullanılan modeldir indüklenen. Bu reaktif olduğundadamarların dış alana uygulanabilir trombositleri ve pıhtılaşma kademeli dizisinin parçalarına dolaşımdaki endotel hücre koruma kaybı, vasküler hücreler 6-8 oksidatif hasarı neden olur. FeCl3 modeli basit ve hem antikoagülan ve anti-trombosit ilaçlara duyarlı olduğunu ve fareler, sıçan, kobay ve tavşan 6-15 yılında karotis ve femoral arterler, şahdamarından ve mezenterik ve kremasterik arteriyollerde ve venüllerden üzerinde yapılmıştır. Bu modelde biri ölçülebilir bir parametre Doppler debimetre ile veya Intravital mikroskopi 6,7,9 doğrudan gözlem altında kan akımının kesilmesi olarak ölçülen damar tıkanıklığı, tamamlamak için yaralanma geçen süredir. 5 ila 30 dakika arasında zaman bir dizi işaret, C57BL6 farelerine 7-10,16 farklı çalışmalarında rapor edildiği FeCl3 konsantrasyonları, anestezi cerrahi teknikler, fare yaş, genomik arka, b, ölçüm yönteminin türleriLood akışı ve diğer çevresel değişkenler, bu modelde anlamlı bir etkiye sahiptir. Bu geniş değişkenlik zor farklı araştırma gruplarından çalışmaları karşılaştırmak ve ince farklılıkların tespiti zor hale getirebilir yapar. Böyle bir değişkenliğe en aza indirmek ve in vivo model sisteminde bir homojen tekrarlanabilir kurmak için bir vizyon ile, minimal değişiklik 6-10,16-19 ile son derece tekrarlanabilir veri yapar FeCl3 indüklenen karotid arter modeli rafine. Bu yazıda tarif ve becerilerini paylaşmak ve bu model yararlanabilir birkaç temsilci deneysel rapor örnekleri.

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			<td height="20" style="height:20px;width:351px;">Surgical Scissors - Tungsten Carbide</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td style="width:119px;">14502-14</td>
			<td style="width:325px;">cut and hold skin</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Micro-Adson Forceps - Serrated/Straight/12cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>11018-12</td>
			<td>cut and hold skin</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:351px;">Metzenbaum Fino Scissors - Tungsten Carbide/Curved/Blunt-Blunt/14.5cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td style="width:119px;">14519-14&#160;&#160;</td>
			<td>to dissect and separate soft tissue</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Ultra Fine Hemostat - Smooth/Curved/12.5cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>13021-12</td>
			<td>to dissect and separate soft tissue</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Graefe Forceps - Serrated/Straight/10cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td style="width:119px;">11050-10</td>
			<td>to dissect and separate soft tissue</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:351px;">Dumont #5 Fine Forceps - Biology Tips/Straight/Inox/11cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>11254-20&#160;</td>
			<td>Isolate vessel from surounding tissue</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:351px;">Dumont #5XL Forceps - Standard Tips/Straight/Inox/15cm</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>11253-10</td>
			<td>Isolate vessel from surounding tissue</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Blunt Hook- 12cm/0.3mm Tip Diameter</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>10062-12</td>
			<td>Isolate vessel from surounding tissue</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Castroviejo Micro Needle Holders</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>12061-02</td>
			<td>Needle holders</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Suture Thread 4-0</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>18020-40</td>
			<td>For fix the incisors to the plate</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Suture Thread 6-0</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>18020-60</td>
			<td>For all surgery and ligation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Kalt Suture Needles</td>
			<td style="width:159px;">Fine Science Tools&#160;</td>
			<td>12050-03</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">rhodamine 6G&#160;</td>
			<td style="width:159px;">Sigma</td>
			<td>83697-1G</td>
			<td>To lebel platelets</td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;">FeCl3 (Anhydrous)</td>
			<td style="width:159px;">Sigma</td>
			<td style="width:119px;">12321</td>
			<td>To induce vessel injury</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Papaverine hydrochloride</td>
			<td style="width:159px;">Sigma</td>
			<td>P3510</td>
			<td>To inhibit gut peristalsis.</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:351px;">Medline Surgical Instrument Sterilization Steam Autoclave Tapes</td>
			<td style="width:159px;">Medline</td>
			<td>111625</td>
			<td>To fix the mouse to the plate</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Fisherbrand&#8482;&#160;Syringe Filters - Sterile 0.22&#181;m</td>
			<td style="width:159px;">Fisher</td>
			<td>09-720-004</td>
			<td>For sterlization of solutions injected to mice</td>
		</tr>
		<tr height="26">
			<td height="26" style="height:26px;width:351px;">Fisherbrand&#8482;&#160;Syringe Filters - Sterile 0.45&#181;m</td>
			<td style="width:159px;">Fisher</td>
			<td>09-719D</td>
			<td>To filter the FeCl3 solution</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Sterile Alcohol Prep Pad</td>
			<td style="width:159px;">Fisher</td>
			<td>06-669-62</td>
			<td>To sterilize the surgical site</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Agarose&#160;</td>
			<td style="width:159px;">BioExpress</td>
			<td>E-3120-500</td>
			<td>To make gel stage</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Leica DMLFS fluorescent microscope</td>
			<td style="width:159px;">Leica</td>
			<td style="width:119px;"></td>
			<td>Intravital microscope</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:351px;">GIBRALTAR Platform and X-Y Stage System</td>
			<td style="width:159px;">npi electronic GmbH</td>
			<td style="width:119px;"></td>
			<td style="width:325px;">http://www.npielectronic.de/products/micropositioners/burleigh/gibraltar.html</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:351px;">Streampix version 3.17.2 software</td>
			<td style="width:159px;">NorPix</td>
			<td style="width:119px;"></td>
			<td>https://www.norpix.com/</td>
		</tr>
	</tbody>
</table>

ferric chloride-induced murine thrombosis models

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, diabetes, obesity, cancer and chronic autoimmune rheumatologic disorders. Thrombi are the cause of most heart attacks, strokes and extremity loss, making thrombosis an extremely important public health problem. Since these thrombi stem from inappropriate platelet activation and subsequent coagulation, targeting these systems therapeutically has important clinical significance for developing safer treatments. Due to the complexities of the hemostatic system, in vitro experiments cannot replicate the blood-to-vessel wall interactions; therefore, in vivo studies are critical to understand pathological mechanisms of thrombus formation. To this end, various thrombosis models have been developed in mice. Among them, ferric chloride (FeCl3) induced vascular injury is a widely used model of occlusive thrombosis that reports platelet activation and aggregation in the context of an aseptic closed vascular system. This model is based on redox-induced endothelial cell injury, which is simple and sensitive to both anticoagulant and anti-platelets drugs. The time required for the development of a thrombus that occludes blood flow gives a quantitative measure of vascular injury, platelet activation and aggregation that is relevant to thrombotic diseases. We have significantly refined this FeCl3-induced vascular thrombosis model, which makes the data highly reproducible with minimal variation. Here we describe the model and present representative data from several experimental set-ups that demonstrate the utility of this model in thrombosis research.

Karotis Arter Trombozu Modeli C57BL6 kökenli farelerde, biz bir başlangıç ​​noktası olarak 1 dakika boyunca gemi tedavisi için% 7.5 FeCl 3 kullanmanızı öneririz. % 7.5 tedavisi FeCl3 altında, yaralı alanı ve normal damar duvarında sınırları kolayca (Bkz online video 1) mikroskop altında tespit edilir, endotel tabakası önemli ölçüde hasar gördü düşündürmektedir. Trombüs FeCl3 tedavi ...

Watch this Scientific Journal Video about Ferric Chloride-induced Murine Thrombosis Models at JoVE.com

Ferric Chloride-induced Murine Thrombosis Models

Biz trombüsler oluşumunu tıkayıcı zaman izlemek için intravital mikroskopi kullanılarak etkin bir şekilde karakterize bir rafine demir klorür (FeCl3) karotis ve mezenterik arter üzerinde indüklenen trombozu modelleri prosedürü yanı sıra damar, rapor.

Ferrik klorit indüklenen fare tromboz modellerinde

Arterial thrombosis (blood clot) is a common complication of many systemic diseases associated with chronic inflammation, including atherosclerosis, ...

Research

JoVE Journal

Medicine

Electrolytic Inferior Vena Cava Model (EIM) of Venous Thrombosis

Electrolytic Inferior Vena Cava Model EIM of Venous Thrombosis

Ven Trombozu Elektrolitik inferior vena kava Modeli (EIM)

Animal models serve a vital role in deep venous thrombosis (DVT) research in order to study thrombus formation, thrombus resolution and to test potential ...

Mouse Complete Stasis Model of Inferior Vena Cava Thrombosis

İnferior Vena Kava Trombozu Fare Komple Durağanlık Model

Venous thromboembolism (VTE) includes both deep vein thrombosis (DVT) and pulmonary embolism (PE).  In the United States (U.S.), the high morbidity and ...

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

Segmentasyon ve Mürin Obezite Modelleri Yağ Hacimleri ölçümü X-ray Bilgisayarlı Tomografi Kullanımı

Obesity is associated with increased morbidity and mortality as well as reduced metrics in quality of life.1 Both environmental and genetic factors are ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

İntraserebral Kanama Fare Modellerinden olarak Otolog Blood veya Klostridyal Kollajenazının intrastriatal Enjeksiyon

Intracerebral hemorrhage (ICH) is a common form of cerebrovascular disease and is associated with significant morbidity and mortality. Lack of effective ...

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

Farelerin Meme Fat Pad içine Meme Kanseri Hücreleri Ortotopik Enjeksiyon Tümör Büyüme Eğitim için.

Breast cancer growth can be studied in mice using a plethora of models. Genetic manipulation of breast cancer cells may provide insights into the ...

Adapting Human Videofluoroscopic Swallow Study Methods to Detect and Characterize Dysphagia in Murine Disease Models

İnsan Videofloroskopik Kırlangıç ​​Eğitim Yöntemleri uyarlanması Algılama ve Mürin Hastalık Modelleri disfajiyi Characterize

This study adapted human videofluoroscopic swallowing study (VFSS) methods for use with murine disease models for the purpose of facilitating ...

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

Fare Servikal lenf nodu Yüksek frekanslı Ultrasonik Görüntüleme

High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal ...

A Multicenter MRI Protocol for the Evaluation and Quantification of Deep Vein Thrombosis

Derin Ven Trombozu Değerlendirilmesi ve Niceleme A Çok Merkezli MRI Protokolü

We evaluated a magnetic resonance venography (MRV) approach with gadofosveset to quantify total thrombus volume changes as the principal criterion for ...

Ferric Chloride-induced Thrombosis Mouse Model on Carotid Artery and Mesentery Vessel

Ferric Klorür kaynaklı Karotis Arter ve Mezenter Gemi Tromboz Fare Modeli

Severe thrombosis and its ischemic consequences such as myocardial infarction, pulmonary embolism and stroke are major worldwide health issues. The ferric ...