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DOI :
10.3791/54492-v
December 16th, 2016
Chapters
0:05
Title
0:47
Selecting Females for Egg Collection via Interrupted Mating
1:31
Preparation of Sperm Solution
2:50
UV Treatment of Sperm Solution
3:37
Manual Extrusion of Mature Eggs
4:56
In Vitro Fertilization
5:51
Heat Shock Treatment
7:13
Selection of Embryos with a One-cycle Cytokinesis Stall
8:48
Results: Heat Shock 2 Treatment of Zebrafish Embryos
9:57
Conclusion
倍数性操作のための修正されたプロトコルは、初期胚に細胞質分裂における1サイクルのストールを誘導するために熱ショックを使用しています。このプロトコルは、ゼブラフィッシュで実証されているが、他の種にも適用可能です。
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