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DOI :
10.3791/54492-v
December 16th, 2016
Chapters
0:05
Title
0:47
Selecting Females for Egg Collection via Interrupted Mating
1:31
Preparation of Sperm Solution
2:50
UV Treatment of Sperm Solution
3:37
Manual Extrusion of Mature Eggs
4:56
In Vitro Fertilization
5:51
Heat Shock Treatment
7:13
Selection of Embryos with a One-cycle Cytokinesis Stall
8:48
Results: Heat Shock 2 Treatment of Zebrafish Embryos
9:57
Conclusion
배수성 조작 수정 된 프로토콜은 초기 배아 세포질 분열에 한 사이클 실속을 유도하는 열 쇼크를 사용한다. 이 프로토콜은 제브라에서 설명되지만, 다른 종에도 적용 할 수있다.
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