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DOI :
10.3791/54508-v
September 28th, 2016
Chapters
0:05
Title
0:53
K. pneumoniae Culture Preparation
2:36
Murine Intratracheal (i.t.) K. pneumoniae Aspiration
3:26
Bronchoalveolar Lavage Fluid (BALF) Collection and Analysis
4:23
Bacterial Lung and Peripheral Tissue Load Determination
5:20
Results: Representative Murine Systemic and Immune Cell Responses to K. pneumoniae Oropharynx Delivery
6:42
Conclusion
いくつかの方法は、マウスにおける細菌性肺炎をモデル化するための文献に記載されています。ここで、我々は、中咽頭にピペット細菌接種の吸引( すなわち 、吸入)を介して肺炎を誘導するための非侵襲性の、安価で迅速な方法を記載します。肺の先天性免疫応答を評価するための下流の方法も詳しく説明されています。
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