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DOI :
10.3791/54518-v
•
10:37 min
December 9th, 2016
Chapters
0:05
Title
0:30
Cell Harvesting and Freezing
3:09
Cryogenic Disruption of Frozen Cells
4:50
Affinity Capture of Protein Complexes from Cell Extracts
6:02
Affinity Capture
7:41
Results: Comparing Some Different Aspects of Affinity Capture Optimization
9:38
Conclusion
这里,我们描述协议破坏由固态研磨哺乳动物细胞在低温下,产生从所得细胞粉细胞提取物,并且在抗体偶联微米级的顺磁珠亲和捕获隔离感兴趣的蛋白质复合物。
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