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DOI :
10.3791/54518-v
•
10:37 min
December 9th, 2016
Chapters
0:05
Title
0:30
Cell Harvesting and Freezing
3:09
Cryogenic Disruption of Frozen Cells
4:50
Affinity Capture of Protein Complexes from Cell Extracts
6:02
Affinity Capture
7:41
Results: Comparing Some Different Aspects of Affinity Capture Optimization
9:38
Conclusion
ここでは、極低温固体ミリングによって、哺乳動物細胞を破壊し、得られた細胞粉末から細胞抽出物を生成し、そして抗体結合ミクロンスケールの常磁性ビーズ上にアフィニティー捕捉により、目的のタンパク質複合体を単離するためのプロトコルを説明します。
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