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DOI :
10.3791/54522-v
September 30th, 2016
Chapters
0:05
Title
0:46
Protoplast Preparation
4:08
Transformation of U. maydis
6:35
Verification of Deletion Events: Preparing gDNA
9:17
Results: Deletion Mutant Experiment
10:43
Conclusion
我们描述了一个强大的基因替代策略,以基因操纵黑粉菌玉米黑粉菌 。该协议说明了如何生成缺失突变体研究感染表型。它可以扩展到修改的基因以任何期望的方式, 例如 ,通过添加编码荧光蛋白标记的序列。
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