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DOI :
10.3791/54527-v
October 23rd, 2016
Chapters
0:05
Title
1:16
Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
3:18
Effect of HdeB Overexpression on E. coli Survival under Acid Stress
5:14
Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
7:16
Conclusion
这项研究说明生物物理,生物化学和分子生物学技术的酸性pH条件下表征大肠杆菌 HdeB的伴侣活性。这些方法已被成功地用于其它酸保护伴侣分子如HdeA蛋白,并且可以进行修改,以用于其它分子伴侣和应力的条件下工作。
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