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DOI :
10.3791/54527-v
October 23rd, 2016
Chapters
0:05
Title
1:16
Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
3:18
Effect of HdeB Overexpression on E. coli Survival under Acid Stress
5:14
Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
7:16
Conclusion
この研究は、酸性pH条件下で大腸菌 HdeBのシャペロン活性を特徴づけるために、生物物理学的生化学的および分子技術を説明しています。これらのメソッドは、成功し、このようなHdeAなどの他の酸保護シャペロンに適用されており、他のシャペロンとストレス条件のために働くように修正することができます。
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