Prosjektet er delvis finansiert av Zentrales Innovationsprogramm Mittelstand (ZIM) des Bundesministeriums für Wirtschaft und Energie -KF3010902AJ4. Publikasjonen avgiften er omfattet av BG traumer sykehuset Tübingen, Tyskland.

MERK: Udødeliggjort human mesenchymale stromal forløperceller (SCP-1 celler) ble anvendt for eksperimentene. SCP-1 celler ble gitt av professor Matthias Schieker 12. 1. Utvidelse av SCP-1 celler <ol><li> Før arbeid med SCP-1 celler, riktig rengjøre arbeidsområdet (betegnet biosikkerhet kabinett jeg) med 70% etanol (v / v) hansker. </li><li> I renset biosikkerhet skapet fremstille et passende volum av cellekulturmediet ved å blande de nødvendige komponenter som angitt i <s...

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	<li>Bridwell, K. H., Anderson, P. A., Boden, S. D., Vaccaro, A. R., Wang, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What's+new+in+spine+surgery.">What's new in spine surgery.</a> J Bone Joint Surg Am. 95, 1144-1150 (2013).</li><li>Adams, M. 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W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Basic+scientific+considerations+in+total+disc+arthroplasty.">Basic scientific considerations in total disc arthroplasty.</a> Spine J. 4, 219-230 (2004).</li><li>Buck, A. E., Kaps, H. -. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implant+for+surgical+use+in+humans+or+vertebrates.">Implant for surgical use in humans or vertebrates.</a> US8728164 B2. Google Patents. , (2014).</li><li>Kettler, A., Kaps, H. P., Haegele, B., Wilke, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biomechanical+behavior+of+a+new+nucleus+prosthesis+made+of+knitted+titanium+filaments.">Biomechanical behavior of a new nucleus prosthesis made of knitted titanium filaments.</a> SAS J. 1, 125-130 (2007).</li><li>Nerurkar, N. L., Elliott, D. M., Mauck, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+design+criteria+for+intervertebral+disc+tissue+engineering.">Mechanical design criteria for intervertebral disc tissue engineering.</a> J Biomech. 43, 1017-1030 (2010).</li><li>Elias, C. N., Lima, J. H. C., Valiev, R., Meyers, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biomedical+applications+of+titanium+and+its+alloys.">Biomedical applications of titanium and its alloys.</a> JOM. 60, 46-49 (2008).</li><li>Hallab, N., Link, H. D., McAfee, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biomaterial+optimization+in+total+disc+arthroplasty.">Biomaterial optimization in total disc arthroplasty.</a> Spine (Phila Pa 1976). 28, 139-152 (2003).</li><li>Gustafsdottir, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiplex+cytological+profiling+assay+to+measure+diverse+cellular+states.">Multiplex cytological profiling assay to measure diverse cellular states.</a> PLoS One. 8, e80999 (2013).</li><li>Bocker, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Introducing+a+single-cell-derived+human+mesenchymal+stem+cell+line+expressing+hTERT+after+lentiviral+gene+transfer.">Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer.</a> J Cell Mol Med. 12, 1347-1359 (2008).</li><li>Ehnert, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transforming+growth+factor+beta1+inhibits+bone+morphogenic+protein+(BMP)-2+and+BMP-7+signaling+via+upregulation+of+Ski-related+novel+protein+N+(SnoN):+possible+mechanism+for+the+failure+of+BMP+therapy.">Transforming growth factor beta1 inhibits bone morphogenic protein (BMP)-2 and BMP-7 signaling via upregulation of Ski-related novel protein N (SnoN): possible mechanism for the failure of BMP therapy.</a> BMC Med. 10, 101 (2012).</li><li>Morgan, S. P., Rose, F. R., Matcher, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Optical Techniques in Regenerative Medicine. , (2013).</li><li>Vielreicher, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Taking+a+deep+look:+modern+microscopy+technologies+to+optimize+the+design+and+functionality+of+biocompatible+scaffolds+for+tissue+engineering+in+regenerative+medicine.">Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine.</a> J R Soc Interface. 10, 20130263 (2013).</li><li>Curtis, A., Wilkinson, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Topographical+control+of+cells.">Topographical control of cells.</a> Biomaterials. 18, 1573-1583 (1997).</li><li>Niu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluorescent+imaging+of+endothelial+cells+in+bioengineered+blood+vessels:+the+impact+of+crosslinking+of+the+scaffold.">Fluorescent imaging of endothelial cells in bioengineered blood vessels: the impact of crosslinking of the scaffold.</a> J Tissue Eng Regen Med. , (2014).</li><li>Chan, B. 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Stillaset flate spiller en viktig rolle i dets interaksjon med omkringliggende vev in vivo for derved å bestemme implantater funksjonell holdbarhet. Således er bio-forenlighet av stillaset studert ved in vitro-analyser ved anvendelse av celler (SCP1 cellelinje), når belagt på stillasene. Mikroskopi teknikker som fungerer godt med tynne og optisk transparente stillaser er dårlig egnet for ikke-gjennomsiktige stillasene for å studere biokompatibilitet. Dette er hovedsak...

Kroniske ryggsmerter er en multifaktoriell sykdom. Interessen for en minimal invasiv behandling for degenerative plate sykdom har vokst siden 1950. Frem til i dag, multisegment fusjon av ryggsøylen er den mest brukte behandling. Siden denne metoden ofte fører til begrensninger i mobiliteten av det berørte segmentet 1,2, utforskning av protesetiden ble en bred interesse. Betydelige fremskritt i total plate erstatning og kjernen erstatning har blitt et godt alternativ til å behandle kroniske ryggsmerter en. Til tross for store fremskritt, har ingen av metodene er klinisk evaluert. De mindre stive nucleus implantater representerer et lovende alternativ til total plate erstatning, forutsatt at ringrommet fibrosus er intakt 3,4. Imidlertid er de for tiden foreliggende nukleus implantater på markedet ofte forbundet med komplikasjoner som forandringer i vertebrale legeme, forvridning, vertikal høyde tap av skiven og than mangel på nødvendige tilhørende mekanisk stivhet 5. For å overvinne de gjeldende ulempene, har en ny kjerne implantat fremstilt av titan strikkede tråder blitt utviklet 6. På grunn av den unike strikket struktur, har denne nyutviklede stillas vist særpregede biomekaniske egenskaper, for eksempel demping funksjon, pore størrelse, lastekapasitet og pålitelighet 7. Tar sikte på å teste biokompatibilitet av denne nye kjernen implantat, avbildet store begrensninger i (optisk) analyseteknikker tilskrives den ikke-transparente natur av implantatet. For å teste den biokompatibilitet, spiller celle-metall interaksjon en fremtredende rolle 8-10. En interaksjon mellom cellene og stillaset er nødvendig for stabilisering og dermed til bedre implantatet integrering i vertssystemet. Imidlertid kan en økende innvekst dybde forandre de mekaniske egenskaper for stillaset. Sikte på å investigate om stillas overflaten gir et utgangspunkt for celleadhesjon, proliferasjon og differensiering, eller hvorvidt metallet påvirker cellenes levedyktighet, er det viktig å feilsøke den vanlige velkjente problemet med å avbilde celler på / i ikke-transparente og opake stillaser. For å overvinne denne begrensningen flere fluorescerende baserte teknikker ble utforsket. Selskaper tilbyr et stort utvalg av fluorophores å visualisere levende celler, cellular avdelinger, eller til spesifikke cellulære tilstander 11. Fluoroforer for dette eksperimentet ble valgt ved hjelp av den elektroniske verktøyet spektral viewer for å best passe vår fluorescerende mikroskop. Den utviklede strategi for analyse av adherente celler oppførsel på / i det ikke-gjennomsiktige strikket titan stillaset omfatter følgende: 1) fluoriserende (green fluorescent protein / GFP) merking av de osteochondro-forløperceller til å tillate sporing av cellene på stillas, 2) måle levedyktighet (mitochondrial aktivitet) av cellene, og 3) å visualisere celle-celle og celle-materiale interaksjoner i stillaset. Fremgangsmåten har den fordel at det lett kan overføres til andre adherente celler og andre ikke-transparent eller ugjennomsiktig stillaset. Videre kan levedyktighet og innvekst mønster overvåkes i løpet av flere dager, slik at det kan anvendes med begrensede mengder av stillasmateriale eller celler. Den foreliggende studien viser vellykket bruk av vår nåværende protokoll for å måle cellenes levedyktighet og visualisere i-vekstmønster av osteochondro-progenitorceller på / i det ikke-gjennomsiktige strikket titan stillaset. Videre kan de industrialiserte protokoller brukes for å bestemme de stillasurenheter og for å kontrollere renseprotokoller.

<table border="0" cellpadding="0" cellspacing="0" width="623">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">6/24/48 well plates, T25/ T75 culture flask</td>
			<td style="width:205px;">Greiner Bio-One GmbH</td>
			<td style="width:144px;">*</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">* 24 well plates</td>
			<td style="width:205px;">Greiner Bio-One GmbH</td>
			<td style="width:144px;">CELLSTAR 662 160</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">* 48 well plates</td>
			<td style="width:205px;">Corning Incorporated USA</td>
			<td style="width:144px;">3548</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">* 6 well plates</td>
			<td style="width:205px;">Falcon</td>
			<td style="width:144px;">353046</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">* T25</td>
			<td style="width:205px;">Greiner Bio-One GmbH</td>
			<td style="width:144px;">690 175</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">* T75</td>
			<td style="width:205px;">Greiner Bio-One GmbH</td>
			<td style="width:144px;">658 175</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Acetic acid, purum &#8805; 99,0 %</td>
			<td style="width:205px;">Carl Roth</td>
			<td style="width:144px;">3738.4</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Acetone</td>
			<td style="width:205px;">Carl Roth</td>
			<td style="width:144px;">5025.1</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Axioplan-2&#160;</td>
			<td style="width:205px;">Carl Zeiss, Germany</td>
			<td style="width:144px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Biological safety cabinets</td>
			<td style="width:205px;">Thermo Scientific</td>
			<td style="width:144px;">safe 2020</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Calcein acetoxymethyl ester (calcein AM)</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">17783</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Cell Culture Incubtator</td>
			<td style="width:205px;">Binder, Tuttlingen, Germany</td>
			<td style="width:144px;">9040-0078</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Filter unit (0.22&#181;m)</td>
			<td style="width:205px;">Millipore, IRL</td>
			<td style="width:144px;">SLGP033RS</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Centrifuges 5810 R And 5417 R</td>
			<td style="width:205px;">Thermo Fisher Scientific, NY</td>
			<td style="width:144px;">Megafuge 40R</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Dimethylsulfoxid (DMSO)</td>
			<td style="width:205px;">Carl Roth</td>
			<td style="width:144px;">4720.2</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Dulbecco&#8217;s PBS without Ca &#38; Mg</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">H15-002</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Ethanol 99 %</td>
			<td style="width:205px;">&#160;SAV liquid prod. GmBH</td>
			<td style="width:144px;">475956</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Ethidium homodimer</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">46043</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">EVOS Fluorescence imaging system</td>
			<td style="width:205px;">Life technologies</td>
			<td style="width:144px;">AMF4300</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Fetal Bovine Serum (FCS)</td>
			<td style="width:205px;">Gibco</td>
			<td style="width:144px;">10270-106</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Hemocytometer</td>
			<td style="width:205px;">Hausser Scientific, PA, USA</td>
			<td style="width:144px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Hoechst 33342</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">14533-100MG</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Knitted titanium nucleus implant</td>
			<td style="width:205px;">Buck co &#38; KG,Germany</td>
			<td style="width:144px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:275px;">MEM Alpha Modification with Glutamine w/o nucleoside</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">E15-832</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Omega microplate Reader</td>
			<td style="width:205px;">BMG Labtech,Germany</td>
			<td style="width:144px;">FLUOstar Omega</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Penicillin/Streptomycin</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">P11-010</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Resazurin sodium salt</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">199303-1G</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Sulforhodamine B sodium salt</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">S1402-1G</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:275px;">Test tube rotator</td>
			<td style="width:205px;">Labinco B.V.,The Netherlands</td>
			<td style="width:144px;">Model LD-76</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">TRIS (hydroxymethyl) aminomethan</td>
			<td style="width:205px;">Carl Roth</td>
			<td style="width:144px;">AE15.1</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Triton</td>
			<td style="width:205px;">Carl Roth</td>
			<td style="width:144px;">3051.2</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Trypan Blue 0.5 %</td>
			<td style="width:205px;">Carl Roth</td>
			<td style="width:144px;">CN76.1</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:275px;">Trypsin/EDTA</td>
			<td style="width:205px;">Sigma</td>
			<td style="width:144px;">L11-004</td>
		</tr>
	</tbody>
</table>

imaging cell viability on non-transparent scaffolds &#8212; using the example of a novel knitted titanium implant

Intervertebral disc degeneration and disc herniation is one of the major causes of lower back pain. Depletion of extracellular matrix, culminating in nucleus pulposus (NP) extrusion leads to intervertebral disc destruction. Currently available surgical treatments reduce the pain but do not restore the mechanical functionality of the spine. In order to preserve mechanical features of the spine, total disc or nucleus replacement thus became a wide interest. However, this arthroplasty era is still in an immature state, since none of the existing products have been clinically evaluated.
This study intends to test the biocompatibility of a novel nucleus implant made of knitted titanium wires. Despite all mechanical advantages, the material has its limits for conventional optical analysis as the resulting implant is non-transparent. Here we present a strategy that describes in vitro visualization, tracking and viability testing of osteochondro-progenitor cells on the scaffold. This protocol can be used to visualize the efficiency of the cleaning protocol as well as to investigate the biocompatibility of these and other non-transparent scaffolds. Furthermore, this protocol can be used to show adherence pattern of cells as well as cell viability and proliferation rates on/in the scaffold. This in vitro biocompatibility testing assay provides a propitious tool to analyze cell-material interaction in non-transparent and opaque scaffolds.

Foreløpige resultater viste at den beskrevne nye kjernen implantatet ikke bare har gode dempe funksjoner, men også er biokompatibelt med SCP-1-celler. Under produksjonsprosessen av implantatet, den kommer i kontakt med sterke etsende og giftige stoffer (smøremiddel, mordant, elektro-polering løsning). Med hjelp av indirekte fluorescerende fargeteknikker var vi i stand til å visualisere de resterende urenheter og dermed optimalisere en renseprotokoll som viser signifikant reduksjon i...

Watch this Scientific Journal Video about Imaging Cell Viability on Non-transparent Scaffolds Ã¢â‚¬â€ Using the Example of a Novel Knitted Titanium Implant at JoVE.com

Imaging Cell Viability on Non-transparent Scaffolds ÃƒÂ¢Ã¢â€šÂ¬Ã¢â‚¬Â Using the Example of a Novel Knitted Titanium Implant

Her presenterer vi en fluorofor basert avbildningsteknikk for å detektere cellenes levedyktighet på en ikke-transparent titan stillaset, så vel som å detektere glimt av stillasurenheter. Denne protokollen feilsøker den ulempe avbildning celle-celle eller celle-interaksjoner metall på ikke-transparente stillasene.

Imaging celleviabilitet på ugjennomsiktige Stillas - Bruke Eksempel på en roman Strikket Titanium Implant

Intervertebral disc degeneration and disc herniation is one of the major causes of lower back pain. Depletion of extracellular matrix, culminating in ...

Research

JoVE Journal

Bioengineering

Imaging Cell Viability on Non-transparent Scaffolds &#8212; Using the Example of a Novel Knitted Titanium Implant

Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Densitetsgradient Flerlags Polymerisasjon (DGMP): En ny metode for å lage flere kammer, som kan tilpasses Stillaser for Tissue Engineering

Complex tissue culture matrices, in which types and concentrations of biological stimuli (e.g. growth factors, inhibitors, or small molecules) or matrix ...

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Wide-field fluoriserende mikroskopi og fluoriserende Imaging flowcytometri på en mobiltelefon

Fluorescent microscopy and flow cytometry are widely used tools in biomedical research and clinical diagnosis. However these devices are in general ...

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Egenrapportering Stillaser for 3-dimensjonal Cell Culture

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting ...

Cell Patterning on Photolithographically Defined 
Parylene-C: SiO2 Substrates

Cell Patterning on Photolithographically Defined 
Parylene-C: SiO2 Substrates

Cell Mønster på Photolithographically Definert Parylene-C: SiO<sub&gt; 2</sub&gt; Underlag

Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain ...

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Levedyktighet Bioprinted Cellular konstruerer Ved hjelp av en tre Enser kartesisk Printer

Tissue engineering has centralized its focus on the construction of replacements for non-functional or damaged tissue. The utilization of ...

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique

Selektiv Cell Elimination fra Mixed 3D Kultur Ved hjelp av en nær-infrarødt Photoimmunotherapy Technique

Recent developments in tissue engineering offer innovative solutions for many diseases. For example, tissue engineering using induced pluripotent stem ...

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Neonatal Cardiac Stillas: Nye matriser for Regenerative Studies

The only definitive therapy for end stage heart failure is orthotopic heart transplantation.  Each year, it is estimated that more than 100,000 donor ...

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Fastsettelse av Zeta Potensielle via nanopartikkel trans Hastigheter gjennom en fleksibel membran nanopore: Ved hjelp av DNA-modifisert Partikler som et eksempel

Nanopore technologies, known collectively as Resistive Pulse Sensors (RPS), are being used to detect, quantify and characterize proteins, molecules and ...

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Fabrikasjon av Decellularized brusk-avledet Matrix stillaser

Osteochondral defects lack sufficient intrinsic repair capacity to regenerate functionally sound bone and cartilage tissue. To this extent, cartilage ...

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Flowcytometri med høy dimensjonalitet for immunfunksjonsanalyse av dissekert implantatvev

The success of implanting laboratory-grown tissue or a medical device in an individual is subject to the immune response of the recipient host. ...