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DOI :
10.3791/54544-v
November 28th, 2016
Chapters
0:05
Title
0:44
Hemolymph Collection to Determine Concentration
1:45
Hemolymph Collection and Immunochemistry
4:32
Larval Lymph Gland Dissection
6:31
Antibody Staining of Larval Lymph Glands
8:32
Mounting Lymph Gland Tissue for Microscopy
9:32
Results: Quantification and Imaging of Hemocytes, and Imaging of Dissected and Immunostained Lymph Gland Tissue
11:19
Conclusion
果蝇和哺乳动物造血系统有许多共同的特点,使果蝇有吸引力的遗传模型来研究造血功能。这里,我们证明了免疫组化的主要造血幼虫器官解剖和安装。我们还描述的方法来测定各种幼虫造血车厢,包括循环血细胞和无柄水晶细胞。
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